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[葡萄糖诱导莱氏无胆甾原体细胞中氢离子、钾离子和乳酸的转运]

[Glucose induced transport of H+, K+ and lactate in the cells of Acholeplasma laidlawii].

作者信息

Kapitanov A B, Ivanov A S, Tarshis M A, Antonov V F

出版信息

Biofizika. 1979 Jan-Feb;24(1):82-6.

PMID:35239
Abstract

Currents of H+ and K+ ions initiated under the effect of facultative anaerobe of A. laidlawii, valinomycin, nigericin and carbonyl cyanide chloride phenylhydrasone on the membrane are recorded by means of the system of cation sensitive electrodes. It is stated that during glucose transport a redistribution of H+ and K+ and lactate between the cellular content and the medium takes place. Preincubation of cells with 3-o-methyl-D-glucose essentially decreases the effect of induced ion transport. It is concluded that ion transport observed in the experiments with glucose is related not to glucose transport through the cell membrane but to the release of its metabolic products from the cells.

摘要

利用阳离子敏感电极系统记录了在赖氏无乳杆菌兼性厌氧菌、缬氨霉素、尼日利亚菌素和羰基氰化物氯苯腙作用下,细胞膜上引发的H⁺和K⁺离子电流。结果表明,在葡萄糖转运过程中,细胞内容物与培养基之间发生了H⁺、K⁺和乳酸的重新分布。用3 - O - 甲基 - D - 葡萄糖对细胞进行预孵育,可显著降低诱导离子转运的效果。得出的结论是,在葡萄糖实验中观察到的离子转运并非与葡萄糖通过细胞膜的转运有关,而是与细胞中代谢产物的释放有关。

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