黑毛钝绥螨 Vitellogenin 基因的分子特征、表达及功能。
Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.
机构信息
Lab of Predatory Mites, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
出版信息
Exp Appl Acarol. 2022 Mar;86(3):343-356. doi: 10.1007/s10493-022-00698-8. Epub 2022 Mar 3.
Vitellogenin (Vg) is an important factor that impacts oocyte maturation, egg formation and embryonic development in Arthropoda. Two orthologs of Vg gene were obtained from the genome of Phytoseiulus persimilis and termed as PpVg1 and PpVg2. Both orthologs belong to the large lipid transfer protein superfamily. The expression of PpVg1 and PpVg2 was low in immatures and male adults, and increased rapidly in female adults after mating, and reached a peak before the first egg was laid (168× and 20.5× the level in virgin females, respectively). When PpVg1 and PpVg2 were interfered with dsRNA, the relative expression decreased by 81.0 and 30.9%, respectively, and 7.8 and 31.4% interfered individuals died within 24 h. Among surviving individuals, ca. 51.1 and 44.8% are infertile. Factors that might be related to expression of Vg genes are also discussed.
卵黄蛋白原（Vg）是节肢动物卵母细胞成熟、卵子形成和胚胎发育的重要因素。从智利小植绥螨基因组中获得了 Vg 基因的两个直系同源物，分别命名为 PpVg1 和 PpVg2。这两个直系同源物都属于大型脂质转移蛋白超家族。PpVg1 和 PpVg2 在若虫和雄性成虫中的表达水平较低，在交配后的雌性成虫中迅速增加，并在第一次产卵前达到峰值（分别是处女雌虫的 168 倍和 20.5 倍）。当用 dsRNA 干扰 PpVg1 和 PpVg2 时，相对表达量分别下降了 81.0%和 30.9%，并且在 24 小时内有 7.8%和 31.4%的干扰个体死亡。在存活的个体中，约有 51.1%和 44.8%是不育的。还讨论了可能与 Vg 基因表达相关的因素。
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