Department of General Practice, The Second Affiliated Hospital of Dalian Medical University, No.467, Zhongshan Road, Dalian, 116027, Liaoning, China.
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi' an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Biochem Genet. 2022 Dec;60(6):2087-2105. doi: 10.1007/s10528-022-10198-4. Epub 2022 Mar 3.
Non-small cell lung cancer (NSCLC) is a serious threaten to human health globally. Circular RNAs (circRNAs) were testified to alter the progression of NSCLC. This work intended to investigate the functional role of circ_0016760 in NSCLC development and the potential mechanism. Expression of circ_0016760, microRNA (miR)-876-3p and NOVA alternative splicing regulator 2 (NOVA2) was determined via quantitative reverse transcription-PCT (qRT-PCR) or western blotting. Cell viability, clonogenicity and apoptosis were assessed by Cell Counting Kit-8 (CCK-8) assay, colony formation assay and flow cytometry, respectively. Transwell assay was performed to examine cell migration and invasion. Western blotting was also conducted to detect the levels of epithelial-to-mesenchymal transition (EMT)-related proteins. Role of circ_0016760 in vivo was evaluated via xenograft model assay. Moreover, the interaction between miR-876-3p and circ_0016760 or NOVA2 was verified by dual-luciferase reporter assay or RNA Immunoprecipitation (RIP) assay. Circ_0016760 and NOVA2 were upregulated, while miR-876-3p expression was decreased in NSCLC tissues and cells. Circ_0016760 depletion suppressed NSCLC cell proliferation and metastasis in vitro, as well as hampered tumor growth in vivo. Circ_0016760 acted as a sponge of miR-876-3p, and miR-876-3p could target NOVA2. Circ_0016760 might play vital roles in NSCLC by regulating miR-876-3p/NOVA2 axis. Circ_0016760 could promote the malignant development of NSCLC through miR-876-3p/NOVA2 axis, at least in part.
非小细胞肺癌（NSCLC）是全球范围内严重威胁人类健康的疾病。环状 RNA（circRNA）已被证明可改变 NSCLC 的进展。本研究旨在探讨 circ_0016760 在 NSCLC 发展中的功能作用及其潜在机制。通过实时定量逆转录-聚合酶链反应（qRT-PCR）或蛋白质印迹法测定 circ_0016760、microRNA（miR）-876-3p 和 NOV 替代剪接调节剂 2（NOVA2）的表达。通过细胞计数试剂盒-8（CCK-8）测定法、集落形成测定法和流式细胞术分别评估细胞活力、克隆形成能力和细胞凋亡。通过 Transwell 测定法检测细胞迁移和侵袭。还通过蛋白质印迹法检测上皮-间充质转化（EMT）相关蛋白的水平。通过异种移植模型试验评估 circ_0016760 在体内的作用。此外，通过双荧光素酶报告基因测定或 RNA 免疫沉淀（RIP）测定验证 miR-876-3p 与 circ_0016760 或 NOVA2 之间的相互作用。circ_0016760 和 NOVA2 在 NSCLC 组织和细胞中上调，而 miR-876-3p 的表达下调。circ_0016760 缺失抑制 NSCLC 细胞在体外的增殖和转移，并抑制体内肿瘤生长。circ_0016760 可作为 miR-876-3p 的海绵，miR-876-3p 可靶向 NOV2。circ_0016760 可能通过调节 miR-876-3p/NOVA2 轴在 NSCLC 中发挥重要作用。circ_0016760 可能通过 miR-876-3p/NOVA2 轴促进 NSCLC 的恶性发展，至少部分如此。
Zotero 插件