Galybin A A, Ermolin G A, Kurmanova L V
Vopr Med Khim. 1986 May-Jun;32(3):136-9.
A test system for quantitative estimation of antibodies towards insulin was developed using the solid phase immunoenzymatic procedure. The test system was calibrated using affinity antibodies towards insulin, obtained after immunosorption of blood plasma of insulin-resistant patient with diabetes. The normal content of autoantibodies, estimated by means of the procedure in human blood serum, constituted 35.2 X 10(-3) +/- 12.4 X 10(-3) g/l. In the patients with diabetes increased content of autoantibodies towards insulin was usually determined--70 X 10(-3)-180 X 10(-3) g/l.
采用固相免疫酶法研制了一种用于定量检测胰岛素抗体的检测系统。该检测系统使用对胰岛素的亲和抗体进行校准，这些抗体是通过对糖尿病胰岛素抵抗患者的血浆进行免疫吸附后获得的。通过该方法测定人血清中自身抗体的正常含量为35.2×10⁻³±12.4×10⁻³g/l。通常在糖尿病患者中测定到胰岛素自身抗体含量增加，为70×10⁻³ - 180×10⁻³g/l。
