转录组和蛋白质组分析揭示了桢楠的干旱适应景观。
Transcriptomic and proteomic analyses uncover the drought adaption landscape of Phoebe zhennan.
机构信息
Institute of Agro-Bioengineering and College of Life Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, Guizhou, China.
Guizhou Academy of Agricultural Sciences, Guizhou Plant Conservation Technology Center, Guiyang, 550006, Guizhou, China.
出版信息
BMC Plant Biol. 2022 Mar 3;22(1):95. doi: 10.1186/s12870-022-03474-3.
BACKGROUND
Phoebe zhennan S.Lee (nanmu) is listed as a threatened tree species in China, whose growth and development, especially during the seedling stage, can be severely limited by drought. Previous studies on nanmu responses to drought stress involved physiological and biochemical analyses, while the molecular mechanisms remained unclear. Therefore, it is of great significance to carry out molecular biology research on the drought resistance of nanmu and reveal the genetic background and molecular regulation mechanism of nanmu drought resistance.
RESULTS
Drought stress enhanced the soluble sugar (SS), free proline(PRO), superoxide anion (O2·-), and hydrogen peroxide (HO) contents as well as the peroxidase (POD) and monodehydroascorbate reductase (MDHAR) activities of nanmu. However, glutathione S-transferase (GST) activity was sensitive to drought stress. Further transcriptomic and proteomic analyses revealed the abundant members of the differentially expressed genes(DEGs) and differentially expressed proteins(DEPs) that were related to phenylpropanoid and flavonoid biosynthesis, hormone biosynthesis and signal transduction, chlorophyll metabolism, photosynthesis, and oxidation-reduction reaction, which suggested their involvement in the drought response of nanmu. These enhanced the osmotic regulation, detoxification, and enzyme-induced and non-enzyme-induced antioxidant ability of nanmu. Moreover, 52% (447/867) of proteins that were up-regulated and 34% (307/892) down-regulated ones were attributed to the increase and decrease of transcription abundance. Transcript up (T) and protein up (P) groups had 447 overlaps, while transcript down (T) and protein down (P) groups had 307 overlaps, accounting for 54% of up and 35% of down-regulated proteins. The lack of overlap between DEGs and DEPs also suggested that post-transcriptional regulation has a critical role in nanmu response to drought.
CONCLUSIONS
Our research results provide significant insights into the regulatory mechanisms of drought stress in nanmu.
背景
桢楠（nanmu）被列为中国受威胁树种，其生长和发育，特别是在幼苗期，可能会受到干旱的严重限制。以前对楠木应对干旱胁迫的研究涉及生理生化分析，而分子机制尚不清楚。因此，开展楠木抗旱性的分子生物学研究，揭示楠木抗旱的遗传背景和分子调控机制具有重要意义。
结果
干旱胁迫提高了楠木的可溶性糖（SS）、游离脯氨酸（PRO）、超氧阴离子（O2·-）和过氧化氢（HO）含量以及过氧化物酶（POD）和单脱氢抗坏血酸还原酶（MDHAR）的活性。然而，谷胱甘肽 S-转移酶（GST）活性对干旱胁迫敏感。进一步的转录组和蛋白质组分析揭示了丰富的差异表达基因（DEGs）和差异表达蛋白（DEPs），这些基因和蛋白与苯丙烷和类黄酮生物合成、激素生物合成和信号转导、叶绿素代谢、光合作用和氧化还原反应有关，表明它们参与了楠木的干旱响应。这些增强了楠木的渗透调节、解毒以及酶诱导和非酶诱导的抗氧化能力。此外，上调的 52%（447/867）和下调的 34%（307/892）蛋白分别归因于转录丰度的增加和减少。转录上调（T）和蛋白上调（P）组有 447 个重叠，而转录下调（T）和蛋白下调（P）组有 307 个重叠，占上调蛋白的 54%和下调蛋白的 35%。差异表达基因和差异表达蛋白之间缺乏重叠也表明，转录后调控在楠木应对干旱胁迫中具有关键作用。
结论
本研究结果为楠木应对干旱胁迫的调控机制提供了重要见解。
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