[胃蛋白酶构象状态对pH值和温度的依赖性]

[Dependence of pepsin conformational states on pH and temperature].

作者信息

Kozlov L V, Meshcheriakova E A, Zavada L L, Efremov E S, Rashkovetskiĭ L G

出版信息

Biokhimiia. 1979 Feb;44(2):339-49.

PMID:35243

Abstract

A conformational behaviour of pepsin depending on pH and temperature was studied by circular dichroism, differential UV-spectroscopy, calorimetry and enzymatic hydrolysis kinetics. A subtile conformational transition of the enzyme accompanied by changes in the physico-chemical and enzymatic properties of the protein was observed within the temperature interval of 15--40 degrees and within the pH range of 1,1--5,6. The range of pepsin heat denaturation was studied. A diagram of pepsin conformational states under different values of pH and temperature was built.

摘要

通过圆二色性、紫外差光谱、量热法和酶促水解动力学研究了胃蛋白酶依赖于pH值和温度的构象行为。在15 - 40摄氏度的温度区间和1.1 - 5.6的pH范围内，观察到该酶伴随着蛋白质理化性质和酶活性的变化发生了微妙的构象转变。研究了胃蛋白酶热变性的范围。构建了不同pH值和温度下胃蛋白酶构象状态图。

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