从小体积血浆中分离人补体成分C3。

Isolation of human complement component C3 from small volumes of plasma.

作者信息

Gresham H D, Matthews D F, Griffin F M

出版信息

Anal Biochem. 1986 May 1;154(2):454-9. doi: 10.1016/0003-2697(86)90014-x.

Abstract

A method for the isolation of 8-10 mg of human C3 from 20 ml of plasma is described. The procedure is simple and rapid with excellent yields in hemolytic activity (74%) and antigenic activity (68.7%). It consists of polyethylene glycol precipitation, DEAE-Sephacel chromatography, and immunoadsorption. The final product is free of contaminating proteins as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The simplicity and speed of this procedure allow for the continual availability of hemolytically active C3.

摘要

本文描述了一种从20毫升血浆中分离出8 - 10毫克人C3的方法。该方法简单快速，溶血活性（74%）和抗原活性（68.7%）的产率极佳。它包括聚乙二醇沉淀、DEAE - 葡聚糖凝胶层析和免疫吸附。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳评估，最终产物不含污染蛋白。该方法的简单性和快速性使得具有溶血活性的C3能够持续获得。