山羊补体系统：溶血试验和主要蛋白的分离。

The complement system of the goat: haemolytic assays and isolation of major proteins.

机构信息

Animal Science Department, Universidad de las Palmas de Gran Canaria, Arucas, Las Palmas, 35413, Spain.

出版信息

BMC Vet Res. 2012 Jun 26;8:91. doi: 10.1186/1746-6148-8-91.

BACKGROUND

The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins.

RESULTS

The commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H.

CONCLUSIONS

Human RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts.

背景

本研究旨在建立一种用于研究羊奶山羊（Capra aegagrus hircus）补体系统的溶血测定法，并对主要的山羊补体系统蛋白进行表征。

结果

用兔抗体致敏的常用绵羊红细胞对山羊血清的裂解不敏感，但人红细胞（RBC）加兔抗体的组合是找到的用于山羊补体测定的最佳选择。开发了一种基于 HEPES 的缓冲液代替经典的巴比妥盐（barbitone）。分离出三种蛋白质：因子 H、C1q 和 C3，并将其与相应的人蛋白进行比较。开发了一种新的亲和层析技术用于分离因子 H。