Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Bioinformatics Laboratory, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
STAR Protoc. 2022 Feb 18;3(1):101179. doi: 10.1016/j.xpro.2022.101179. eCollection 2022 Mar 18.
Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).
聚糖是存在于活细胞表面的结构多样的分子。该方案详细介绍了一种用于使用人诱导多能干细胞 (hiPSC) 和 hiPSC 衍生的神经祖细胞 (NPC) 对单个细胞中的聚糖和 RNA 进行联合分析的系统 (scGR-seq)。scGR-seq 由基于 DNA 编码的凝集素的测序聚糖分析 (scGlycan-seq) 和单细胞转录组分析 (scRNA-seq) 组成。scGR-seq 将是描绘多细胞系统中糖基细胞异质性的重要技术。有关此配置文件的使用和执行的完整详细信息，请参阅 Minoshima 等人。(2021)。
