Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
JST PRESTO, Tsukuba, Ibaraki, Japan.
Curr Protoc. 2023 May;3(5):e777. doi: 10.1002/cpz1.777.
Single-cell multi-omics analysis has emerged as a crucial tool in modern biology due to its capacity to uncover the intricate molecular heterogeneity within individual cells. Glycan structural diversity on the cell surface creates a "cell signature" that varies by cell type and state. However, single-cell glycan analysis remains a challenge. This protocol presents a series of techniques and procedures that enable the simultaneous measurements of glycan and RNA at the single-cell level via DNA-barcoded lectin-based sequencing (scGR-seq). The techniques include (1) the preparation of DNA-barcoded lectins, (2) a step-by-step protocol for single-cell glycan/RNA sequencing, and (3) a data analysis approach for the integration of glycan and RNA data. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Preparation of DNA-barcoded lectins Basic Protocol 2: Single-cell glycan/RNA sequencing.
单细胞多组学分析由于能够揭示单个细胞内复杂的分子异质性，已成为现代生物学的重要工具。细胞表面糖链结构的多样性形成了一种“细胞特征”，因细胞类型和状态而异。然而，单细胞糖链分析仍然具有挑战性。本方案介绍了一系列技术和步骤，通过基于 DNA 编码的凝集素的测序（scGR-seq）实现了单细胞水平上糖链和 RNA 的同时测量。这些技术包括：（1）DNA 编码凝集素的制备；（2）单细胞糖链/RNA 测序的分步方案；（3）糖链和 RNA 数据整合的数据分析方法。© 2023 威利父子公司。基本方案 1：DNA 编码凝集素的制备基本方案 2：单细胞糖链/RNA 测序。
