一种假定的短链脱氢酶Rv0148影响细菌的存活和毒力。

A putative short-chain dehydrogenase Rv0148 of affects bacterial survival and virulence.

作者信息

Bhargavi Gunapati, Singh Amit Kumar, Patil Shripad A, Palaniyandi Kannan

机构信息

ICMR-National Institute for Research in Tuberculosis, Chennai, India.

ICMR- National JALMA Institute for Leprosy & Other Mycobacterial Diseases, Agra, India.

出版信息

Curr Res Microb Sci. 2022 Feb 12;3:100113. doi: 10.1016/j.crmicr.2022.100113. eCollection 2022.

During infection, combats the stress generated by the host cells through the action of short-chain dehydrogenases/reductases (SDRs). Rv0148 belongs to the oxidoreductase family with the SDRs domain, which regulates the homeostasis of In our earlier studyusing knockout mutant strain (∆0148), we reported that Rv0148 is involved in intermediary metabolism, drug resistance and cell homeostasis of . In the current study, we explored the functional role of Rv0148 using gene knockout mutant and models of infection. We report the ∆0148 is attenuated for virulence of During human monocyte (THP-1) cell line infection, Δ0148 displayed reduced intracellular survival compared to the wild type at successive time points. Similarly, in a guinea pig animal model of aerosol infection, Δ0148 displayed a growth attenuation at 5- and 10-week post-infection in the lungs and spleen compared to the wild-type and Rv0148-complemented Δ0148 strains. Our study suggest that Rv0148 has a distinct role in the intracellular virulence of

在感染过程中，通过短链脱氢酶/还原酶（SDRs）的作用对抗宿主细胞产生的应激。Rv0148属于具有SDRs结构域的氧化还原酶家族，其调节……的稳态。在我们早期使用基因敲除突变株（∆0148）的研究中，我们报道Rv0148参与……的中间代谢、耐药性和细胞稳态。在当前研究中，我们使用基因敲除突变体和感染模型探究了Rv0148的功能作用。我们报道∆0148的毒力减弱。在人单核细胞（THP-1）细胞系感染期间，与野生型相比，∆0148在连续时间点的细胞内存活率降低。同样，在气溶胶感染的豚鼠动物模型中，与野生型和Rv0148互补的∆0148菌株相比，∆0148在感染后5周和10周时在肺和脾中的生长减弱。我们的研究表明Rv0148在……的细胞内毒力中具有独特作用。