Gnezditskaia E V, Beletskaia L V, Ippolitov I Kh, Shagal D I
Biull Eksp Biol Med. 1978 Jun;85(6):709-12.
Deposits of granular material containing IgM, IgA, and IgG were revealed in the thymus of patients with myasthenia by direct immunofluorescence. Treatment of the thymus sections by unlabeled preparations against individual classes of human immunoglobulins inhibited the reaction of the granular material with the homologous labeled preparations. Disappearance of fluorescence of these deposits was also seen in treatment of the sections with glycine-HCl-buffer, pH 2.8. These data permitted a suggestion that granular material represented immune complexes where IgM, IgA, and IgG served as antibody, and thymus tissue components--as an antigen. The presence of bound immunoglobulin in the thymus indicated that an autoimmune process directed against the tissues of this organ occurred in myasthenia.
通过直接免疫荧光法在重症肌无力患者的胸腺中发现了含有IgM、IgA和IgG的颗粒物质沉积物。用针对人类免疫球蛋白各类型的未标记制剂处理胸腺切片，可抑制颗粒物质与同源标记制剂的反应。用pH 2.8的甘氨酸 - 盐酸缓冲液处理切片时，也可见这些沉积物的荧光消失。这些数据提示颗粒物质代表免疫复合物，其中IgM、IgA和IgG作为抗体，胸腺组织成分作为抗原。胸腺中存在结合免疫球蛋白表明重症肌无力患者发生了针对该器官组织的自身免疫过程。
