Groupe de Recherche en Biologie Végétale (GRBV), Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada.
Physiol Plant. 2022 Mar;174(2):e13658. doi: 10.1111/ppl.13658.
Protein carbonylation is a post-translational modification associated with the reactive oxygen species. It results from the direct oxidation of the side chains of Lys, Arg, Pro, and Thr residues by hydroxyl radical HO or the addition of reactive carbonyl species including α,β-unsaturated aldehydes and oxylipins to the side chain of Cys, His, and Lys. Recent findings indicated that the phytohormone abscisic acid (ABA) induces the production of α,β-unsaturated aldehydes that modulate the effect of ABA on stomatal closure. This indicated that α,β-unsaturated aldehydes might mediate ABA signaling. In this study, we investigated the ABA-induced protein carbonylation events by profiling the carbonylated proteome extracted from Arabidopsis thaliana leaves after ABA treatment. The carbonylated proteins were enriched by affinity chromatography and subjected to liquid chromatography-tandem mass spectrometry. We identified 180 carbonylated proteins. Of these, 26 proteins became carbonylated upon ABA treatment, whereas 163 proteins that were carbonylated in untreated samples were no longer detected in the ABA-treated samples, which points to dynamic control of protein carbonylation by ABA in A. thaliana. A few regulatory stress-related proteins and enzymes involved in the biosynthesis of the aspartate family of amino acids were overrepresented in the list of proteins, which the carbonylation status changed between untreated and ABA-treated samples. These results indicated that ABA triggers a change in the pattern of protein carbonylation in A. thaliana. This change is independent of the commonly seen increased levels of carbonylated proteins in the plants subjected to deadly stress conditions.
蛋白质羰基化是一种与活性氧物质相关的翻译后修饰。它是由羟自由基 HO 直接氧化赖氨酸、精氨酸、脯氨酸和苏氨酸残基的侧链，或者由活性羰基物质（包括α，β-不饱和醛和氧化脂类）与半胱氨酸、组氨酸和赖氨酸的侧链加成而产生的。最近的研究结果表明，植物激素脱落酸（ABA）诱导产生α，β-不饱和醛，调节 ABA 对气孔关闭的作用。这表明α，β-不饱和醛可能介导 ABA 信号。在这项研究中，我们通过分析 ABA 处理后拟南芥叶片中提取的羰基化蛋白质组，研究了 ABA 诱导的蛋白质羰基化事件。用亲和层析富集羰基化蛋白质，并进行液相色谱-串联质谱分析。我们鉴定了 180 种羰基化蛋白质。其中，26 种蛋白质在 ABA 处理后发生羰基化，而在未处理样品中羰基化的 163 种蛋白质在 ABA 处理样品中不再被检测到，这表明 ABA 在拟南芥中对蛋白质羰基化进行动态控制。在未处理和 ABA 处理样品之间，一些与调节应激相关的蛋白质和参与天冬氨酸族氨基酸生物合成的酶的羰基化状态发生了变化，这些蛋白质在列表中被过度表示。这些结果表明，ABA 触发了拟南芥中蛋白质羰基化模式的变化。这种变化与植物在受到致命应激条件下常见的羰基化蛋白质水平升高无关。
