Bloom G S, Luca F C, Collins C A, Vallee R B
Ann N Y Acad Sci. 1986;466:328-39. doi: 10.1111/j.1749-6632.1986.tb38404.x.
We have used a taxol-based microtubule purification procedure and monoclonal antibodies to isolate and characterize the MAPs of mitotic spindle microtubules in the fertilized sea urchin egg. In so doing, we hope to have identified some of the essential working parts of the mitotic apparatus, namely those proteins that regulate the assembly, disassembly, organization and mechanochemical properties of spindle microtubules. The results of this effort strongly suggest that a rich diversity of polypeptides associate with mitotic spindle microtubules. Whether each of these represents an individual protein species is not currently known. It is possible, for example, that particular spindle MAPs comprise multiple, distinct subunits. This would not be surprising in light of the facts that both MAP-1 and MAP-2 contain lower molecular weight subunits, and that axonemal dyneins are complex assemblies of several polypeptide species. Our future efforts with the sea urchin system will be to determine how the various mitotic spindle MAPs we have identified function individually and in concert, and how those functions contribute to the mechanochemical properties of the spindle.
我们采用了一种基于紫杉醇的微管纯化方法和单克隆抗体，来分离和鉴定受精海胆卵有丝分裂纺锤体微管的微管相关蛋白（MAPs）。通过这样做，我们希望能够识别出有丝分裂装置的一些关键组成部分，即那些调节纺锤体微管组装、拆卸、组织和机械化学性质的蛋白质。这项工作的结果有力地表明，有多种多肽与有丝分裂纺锤体微管相关联。目前尚不清楚这些多肽是否各自代表一种单独的蛋白质种类。例如，有可能特定的纺锤体微管相关蛋白包含多个不同的亚基。鉴于微管相关蛋白-1（MAP-1）和微管相关蛋白-2（MAP-2）都含有较低分子量的亚基，以及轴丝动力蛋白是几种多肽种类的复杂组装体，这并不奇怪。我们未来在海胆系统上的工作将是确定我们所识别出的各种有丝分裂纺锤体微管相关蛋白如何单独发挥作用以及协同发挥作用，以及这些功能如何对纺锤体的机械化学性质产生影响。
