Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia BO, Italy.
Department of Veterinary Sciences, University of Parma, Strada del Taglio, 10, 43126, Parma, Italy.
J Vet Cardiol. 2022 Jun;41:57-69. doi: 10.1016/j.jvc.2022.01.008. Epub 2022 Feb 4.
INTRODUCTION/OBJECTIVES: Urine chemistry has received growing attention to estimate the diuretic response in dogs with cardiac disease. The aim of the study was to evaluate the impact of time elapsed between the oral furosemide administration and sample collection on urine chemistry in dogs with myxomatous mitral valve disease (MMVD) receiving diuretic therapy in American College of Veterinary Internal Medicine (ACVIM) stage C.
Seventy-three dogs with MMVD ACVIM stage C and 106 healthy dogs were prospectively included. Dogs with MMVD were divided, based on the time of sampling, in morning group (MMVD-MG) of one to 6 h and an evening group (MMVD-EG) over 6 h from oral furosemide administration. Analogously, healthy dogs sampled between 9 a.m. and 1 p.m. and between 2 and 7 p.m. were divided in a morning group (H-MG) and an evening group (H-EG), respectively. Urine chemistry, including fractional excretion of electrolytes, was evaluated and compared among groups.
Higher excretion of sodium and chloride and higher urine sodium to urine potassium ratio (uNa:uK) were detected in MMVD-MG than MMVD-EG (P = 0.021, P = 0.038, and P = 0.016, respectively). Natriuresis, chloriuresis, and uNa:uK+ were higher in MMVD-MG than H-MG, while no differences were found in the comparison between H-MG and H-EG and between MMVD-EG and H-EG.
Urinary electrolyte excretion is significantly increased within 6 h from furosemide administration in MMVD ACVIM stage C dogs. Time of sampling from furosemide administration significantly affects urine chemistry in MMVD dogs and should be considered in clinical practice and the research field.
简介/目的：尿液化学分析越来越受到关注，以评估心脏病犬的利尿反应。本研究旨在评估在接受美国兽医内科学院（ACVIM）C 级利尿治疗的黏液样二尖瓣病变（MMVD）犬中，从口服呋塞米给药到采集样本之间的时间间隔对尿液化学的影响。
前瞻性纳入 73 只 MMVD ACVIM C 级犬和 106 只健康犬。根据采样时间，MMVD 犬分为上午组（MMVD-MG），即口服呋塞米后 1-6 小时，和下午组（MMVD-EG），即口服呋塞米后 6 小时以上。同样，健康犬在上午 9 点至下午 1 点和下午 2 点至下午 7 点之间采样，分为上午组（H-MG）和下午组（H-EG）。评估并比较各组的尿液化学，包括电解质的排泄分数。
与 MMVD-EG 相比，MMVD-MG 中钠和氯的排泄量更高，尿钠/尿钾比值（uNa:uK）更高（P=0.021，P=0.038，P=0.016）。与 H-MG 相比，MMVD-MG 中的钠排泄、氯排泄和 uNa:uK+更高，而在 H-MG 与 H-EG 之间以及 MMVD-EG 与 H-EG 之间则无差异。
在 MMVD ACVIM C 级犬中，从呋塞米给药到采集样本的 6 小时内，尿电解质排泄显著增加。从呋塞米给药到采集样本的时间显著影响 MMVD 犬的尿液化学，应在临床实践和研究领域中考虑。
