Institute of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, ul. Ciszewskiego 8, 02-786 Warsaw, Poland.
Institute of Animal Sciences, Warsaw University of Life Sciences - SGGW, ul. Ciszewskiego 8, 02-786 Warsaw, Poland.
Ann Parasitol. 2021;67(4):619-626. doi: 10.17420/ap6704.378.
Tapeworm infections in Konik Polski horses from Biebrza National Park were investigated in this study. Faecal samples were collected 10 times: in 2012 - 1 time, in 2013 - 4 times, in 2014 - 4 times and in 2015 - 1 time. In total, 162 faecal samples were collected and tested. Faecal egg counts (FECs) method was used in the study. Positive samples with cestode eggs were noted only twice - in October 2012 and December 2013 in two adult mares (9 and 11 years old). The determined prevalence was surprisingly low comparing to other studies, 4.3% in October 2013 and 28.5% in December 2013. Parasite genomic DNA was isolated from posterior proglottids of tapeworms found in horse faeces after deworming, PCR technique was applied in order to determine the species of these tapeworms. No specific products were obtained using primers specific to rDNA sequence, whereas, a 620 bp fragment encoding mitochondrial cytochrome oxidase I (COI) was amplified and sequenced. Nucleotide sequence analysis revealed a 100% homology to a corresponding fragment form Anoplocephala perfoliata tapeworm originating from a horse from the Czech Republic. To our knowledge this is the first report applying molecular techniques to tapeworm identification in Konik Polski horses.
本研究调查了比亚沃维耶扎国家公园的波兰原野马的绦虫感染情况。共采集了 162 份粪便样本，采集时间分别为 2012 年 1 次、2013 年 4 次、2014 年 4 次和 2015 年 1 次。研究中使用了粪便虫卵计数（FEC）法。仅在 2012 年 10 月和 2013 年 12 月的两次成年母马（9 岁和 11 岁）粪便中发现了带有绦虫卵的阳性样本。与其他研究相比，确定的流行率出人意料地低，2013 年 10 月为 4.3%，2013 年 12 月为 28.5%。从驱虫后马粪便中发现的绦虫后体节中分离寄生虫基因组 DNA，应用 PCR 技术确定这些绦虫的种类。使用针对 rDNA 序列的特异性引物未获得特异性产物，然而，扩增并测序了编码线粒体细胞色素氧化酶 I（COI）的 620 bp 片段。核苷酸序列分析显示，与源自捷克共和国马的相应片段完全同源。据我们所知，这是首次应用分子技术鉴定波兰原野马的绦虫。
