Department of Virology, Immunology and Parasitology, National Veterinary Institute, Uppsala, Sweden.
Vet Parasitol. 2013 Nov 8;197(3-4):580-5. doi: 10.1016/j.vetpar.2013.07.020. Epub 2013 Jul 22.
A case-control study was performed to investigate the association between colic of all types in Swedish horses and infection with the equine tapeworm Anoplocephala perfoliata. Colic cases were defined by clinical signs consistent with the presence of abdominal pain, and the control horses had no signs of colic within the last year but attended a clinic for other reasons. Blood and fecal samples were collected by veterinarian from 67 horses with signs of colic and 67 control horses. The sera were analyzed using serodiagnostic assay anti-12/13 kDa IgG(T) ELISA. The fecal samples, 30 g from each horse, were analyzed with a modified sugar salt flotation method with a density of 1.280. A significant association was found between the presence of A. perfoliata eggs in feces and colic with a 16 times higher risk of colic if eggs had been observed in fecal samples. However, there was no significant association between colic and the median OD-values in the serological diagnosis, nor when recommended cut-offs were used. The study concludes that A. perfoliata is a risk factor for colic in Swedish horses and it suggests that the modified flotation method can be used as a diagnostic tool for identifying horses at risk.
一项病例对照研究旨在探讨瑞典马的各种绞痛与马绦虫（Anoplocephala perfoliata）感染之间的关联。绞痛病例的定义是符合腹痛存在的临床症状，而对照马匹在过去一年中没有绞痛迹象，但因其他原因就诊于诊所。兽医从 67 匹出现绞痛症状的马和 67 匹对照马中采集了血液和粪便样本。使用抗 12/13 kDa IgG(T)ELISA 血清诊断检测分析血清。每个马的 30 克粪便样本使用改良的糖盐漂浮法进行分析，密度为 1.280。发现粪便中存在 A. perfoliata 卵与绞痛之间存在显著关联，如果粪便样本中观察到卵，则绞痛的风险增加 16 倍。然而，在血清学诊断中，中位数 OD 值之间没有显著关联，也没有使用推荐的截断值。该研究得出结论，A. perfoliata 是瑞典马绞痛的危险因素，并表明改良的漂浮法可作为识别风险马的诊断工具。
