一个包含 MYB109-ZmCesA5 的新网络参与了核的发育。

A new network containing MYB109-ZmCesA5 is involved in kernel development.

机构信息

Maize Research Institute, Southwest University, Tiansheng Road, No. 2, Beibei District, Chongqing, China.

出版信息

Biochem Biophys Res Commun. 2022 Apr 30;602:15-20. doi: 10.1016/j.bbrc.2022.02.104. Epub 2022 Feb 28.

DOI:10.1016/j.bbrc.2022.02.104

Abstract

MYB genes regulate several different aspects of metabolism and development. However, few studies have reported the involvement of MYBs-CesAs network in the regulation of maize kernel development. In this study, yeast one-hybrid (Y1H) assays and dual-luciferase reporter assays showed that ZmMYB109 activated the expression of ZmCesA5 by directly binding to its promoter. Real-time quantitative PCR (RT-qPCR) and transcriptome analyses showed that ZmMYB109 expression increased in ZmCesA5-OE kernels and decreased in ZmCesA5-KO kernels. Overexpression of ZmCesA5 produced heavier kernels, whereas loss of function of ZmCesA5 affected starch and sucrose metabolism, resulting in weight reduction of the maize kernels. Collectively, these findings suggest that a new network containing MYB109-ZmCesA5 is involved in kernel development.

摘要

MYB 基因调控代谢和发育的几个不同方面。然而，很少有研究报道 MYB-CesAs 网络在调节玉米籽粒发育中的作用。在这项研究中，酵母单杂交 (Y1H) 分析和双荧光素酶报告基因分析表明，ZmMYB109 通过直接结合其启动子激活 ZmCesA5 的表达。实时定量 PCR (RT-qPCR) 和转录组分析表明，ZmMYB109 在 ZmCesA5-OE 核中表达增加，在 ZmCesA5-KO 核中表达减少。ZmCesA5 的过表达产生了较重的核，而 ZmCesA5 的功能丧失影响了淀粉和蔗糖代谢，导致玉米核重量减轻。总之，这些发现表明，包含 MYB109-ZmCesA5 的新网络参与了核的发育。