Jordan V C, Jacobson H I, Keenan E J
Cancer Res. 1986 Aug;46(8 Suppl):4237s-4240s.
Three laboratories compared their routine steroid binding assays with the Abbott estrogen receptor-enzyme immunoassay (ER-EIA) to determine ER in breast cancer cytosols. Each laboratory was first trained to use the ER-EIA kit and then performed routine proficiency panels to determine assay reproducibility. The frozen panels prepared from MCF-7 cytosol produced good intraassay results but the between assay coefficients of variation were frequently above 10%. Lyophilized MCF-7 cytosols produced better reproducibility upon repeated assay. One laboratory demonstrated that New England Nuclear steroid binding kits and the ER-EIA produced comparable results when MCF-7 lyophilized cytosols were used. The analysis of breast tumor samples demonstrated excellent linear correlation coefficients for each laboratory (greater than 0.92 N approx. 60 samples each) but different slopes. The comparison of the ER-EIA with the New England Nuclear steroid binding assay produced a slope of 1.13. The ER-EIA appears to produce comparable results to the conventional steroid binding assays for the determination of ER in breast tumor cytosols.
三个实验室将他们的常规类固醇结合测定法与雅培雌激素受体酶免疫测定法（ER-EIA）进行比较，以测定乳腺癌细胞溶质中的雌激素受体（ER）。每个实验室首先接受使用ER-EIA试剂盒的培训，然后进行常规能力验证试验组以确定测定的可重复性。由MCF-7细胞溶质制备的冷冻试验组产生了良好的批内结果，但批间变异系数经常超过10%。冻干的MCF-7细胞溶质在重复测定时产生了更好的可重复性。一个实验室证明，当使用冻干的MCF-7细胞溶质时，新英格兰核公司的类固醇结合试剂盒和ER-EIA产生了可比的结果。对乳腺肿瘤样本的分析表明，每个实验室的线性相关系数都非常出色（大于0.92，每个实验室约60个样本），但斜率不同。ER-EIA与新英格兰核公司类固醇结合测定法的比较产生的斜率为1.13。对于测定乳腺肿瘤细胞溶质中的ER，ER-EIA似乎能产生与传统类固醇结合测定法可比的结果。
