Bora Mayuri, Sarmah Neelanjana, Das Bhaswatee, Narayan Baruah Munindra, Deka Gitanjali, Gogoi Hazarika Sumita, Baruah Shashi
Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Sonitpur, Assam 784028, India.
North East Cancer Hospital & Research Institute (NECHRI), Jorabat, Guwahati, Assam 781023, India.
Hum Immunol. 2022 May;83(5):453-457. doi: 10.1016/j.humimm.2022.02.006. Epub 2022 Mar 2.
The expression of immunomodulatory molecule HLA-G is tissue restricted with abundant expression in placenta, mediating immune tolerance to fetus. Tumors hijack HLA-G to establish nutrient supply and evade host immune response. 14 base pair Insertion/Deletion polymorphism (rs371194629) and + 3142 G/C SNP (rs1063320) of 3'UTR of HLA-G were investigated in conjunction with miR-148A, miR-152 and HLA-G expression in SAB (Spontaneous abortion) history placenta and HNSCC tumor in a hospital-based case control study. Higher frequency of G allele of rs1063320 was seen in study participants as reported in other global populations. Both miR-148A and miR-152 were downregulated in tumor tissue. Predominance of 14 base pair "IN" allele of rs371194629 was noted in SAB placental tissue (p =<0.0001) with lower expression of HLA-G levels. In conclusion, 14 base pair Insertion/Deletion in linkage with + 3142 G/C SNP was related to lower HLA-G protein expression in SAB tissue, contradictorily HLA-G protein level was manipulated by tumors by suppressing microRNAs.
免疫调节分子HLA - G的表达具有组织限制性，在胎盘中大量表达，介导对胎儿的免疫耐受。肿瘤利用HLA - G来建立营养供应并逃避宿主免疫反应。在一项基于医院的病例对照研究中，研究了HLA - G 3'UTR的14个碱基对插入/缺失多态性（rs371194629）和 +3142 G/C SNP（rs1063320），并结合miR - 148A、miR - 152以及HLA - G在有自然流产（SAB）史的胎盘和头颈部鳞状细胞癌（HNSCC）肿瘤中的表达情况。如在其他全球人群中所报道的那样，研究参与者中rs1063320的G等位基因频率较高。miR - 148A和miR - 152在肿瘤组织中均下调。在SAB胎盘组织中，rs371194629的14个碱基对“IN”等位基因占优势（p =<0.0001），且HLA - G水平较低。总之，与 +3142 G/C SNP连锁的14个碱基对插入/缺失与SAB组织中较低的HLA - G蛋白表达相关，矛盾的是，肿瘤通过抑制微小RNA来调控HLA - G蛋白水平。
