Minakata K, Asano M, Yonekawa O
Clin Biochem. 1986 Jun;19(3):171-4. doi: 10.1016/s0009-9120(86)80018-2.
New proteinase inhibitors have recently been found in seminal plasma which specifically inhibit cysteine proteinases such as ficin, papain, cathepsin H and cathepsin B. The inhibitors consist of two components, In-A and In-B, both of which are effective in native semen. In the present work, a method of assay is devised to determine activities of both components separately. Under conditions in which In-B is denatured completely while In-A is not, inhibitory activity of In-A alone is determined. The mean In-A inhibitory level is 1.52 +/- 0.28 inhibitor unit/mL and the level is independent of the number of spermatozoa. In-B level is calculated by subtracting In-A level from the total ficin inhibition level of the semen. The mean In-B level is 1.47 +/- 0.52 inhibitor unit/ml. In-B level has a tendency to increase with increasing number of spermatozoa. The correlation coefficient is 0.307 and statistical significance p less than 0.005.
最近在精浆中发现了新的蛋白酶抑制剂，它们能特异性抑制半胱氨酸蛋白酶，如无花果蛋白酶、木瓜蛋白酶、组织蛋白酶H和组织蛋白酶B。这些抑制剂由两种成分组成，即In - A和In - B，二者在天然精液中均有效。在本研究中，设计了一种测定方法来分别测定两种成分的活性。在In - B完全变性而In - A未变性的条件下，测定单独In - A的抑制活性。In - A的平均抑制水平为1.52±0.28抑制剂单位/毫升，且该水平与精子数量无关。In - B的水平通过从精液的总无花果蛋白酶抑制水平中减去In - A的水平来计算。In - B的平均水平为1.47±0.52抑制剂单位/毫升。In - B的水平有随精子数量增加而升高的趋势。相关系数为0.307，统计学显著性p小于0.005。
