Wong T, Morgan T O, Alcorn D, Ryan G B
Clin Exp Pharmacol Physiol. 1986 Apr;13(4):267-70. doi: 10.1111/j.1440-1681.1986.tb00346.x.
Active and inactive renin were measured in individual juxtaglomerular apparatus (JGA) and in whole kidney homogenates. The morphology of the JGA was examined in microbiopsy glomerular specimens and in kidneys fixed by arterial perfusion. In rats on high and normal salt intake the total renin content of a single JGA was 14 (s.e.m. = 3) and 29 (s.e.m. = 4) ng AI/h, respectively. The amount of cystoplasm occupied by renin granules was 20% and 27%. Crystalline cores were seen in 1.5% and 7% of the granules, respectively. Increased delivery of NaCl to the macula densa did not alter total renin, but decreased inactive renin from 30% to 0, crystalline core-containing cells from 33% to 14% and decreased the percentage of granules with crystalline cores from 12% to 2.2%. Increased sodium in the diet and increased delivery of NaCl to the macula densa decreased the proportion of renin present in the inactive form and decreased the proportion of crystalline cores. These coincidental alterations suggest that crystalline cores contain inactive renin and suggest that the delivery of sodium to the macula densa activates renin.
分别在单个肾小球旁器（JGA）和全肾匀浆中测定活性肾素和非活性肾素。通过微生物活检肾小球标本和经动脉灌注固定的肾脏来检查JGA的形态。在高盐和正常盐摄入的大鼠中，单个JGA的总肾素含量分别为14（标准误=3）和29（标准误=4）ng AI/h。肾素颗粒占据的细胞质量分别为20%和27%。分别在1.5%和7%的颗粒中可见结晶核心。向致密斑增加NaCl的输送并未改变总肾素，但使非活性肾素从30%降至0，含结晶核心的细胞从33%降至14%，并使含结晶核心的颗粒百分比从12%降至2.2%。饮食中钠的增加以及向致密斑增加NaCl的输送降低了以非活性形式存在的肾素比例，并降低了结晶核心的比例。这些同时发生的改变表明结晶核心包含非活性肾素，并表明向致密斑输送钠可激活肾素。
