School of Life Sciences, Qufu Normal University, Jining, 273 165, China.
Shandong Freshwater Fisheries Research Institute, Jinan, 250013, China.
Environ Sci Pollut Res Int. 2022 Jul;29(34):51665-51673. doi: 10.1007/s11356-022-19516-0. Epub 2022 Mar 5.
Submerged macrophytes and microbial communities are important parts of lake ecosystems. In this study, the bacterial community composition in rhizosphere sediments and water from areas cultivated with (PL) and without (CK) shining pondweed (Potamogeton lucens Linn.) was investigated to determine the effects of P. lucens Linn. on the structure of the bacterial communities in Nansi Lake, China. Molecular techniques, including Illumina MiSeq and qPCR targeting of the 16S rRNA gene, were used to analyze the composition and abundance of the bacterial community. We found that bacterial alpha diversity was higher in PL water than in CK water, and the opposite trend was observed in sediment. In addition, 16S rRNA gene copy number in sediment was lower in PL than in CK. We found 30 (e.g., Desulfatiglans) and 29 (e.g., Limnohabitans) significantly different genera in sediment and water, respectively. P. lucens Linn. can change chemical properties in sediment and water and thereby affect the bacterial community. At the genus level, members of bacterial community clustered according to source (water/sediment) and area (PL/CK). Our study demonstrated that submerged macrophytes can affect the bacterial community composition in both sediment and water, suggesting that submerged macrophytes affect the transportation and cycling of nutrients in lake ecosystems.
沉水植物和微生物群落是湖泊生态系统的重要组成部分。本研究调查了中国南四湖有（PL）和无（CK）菹草（Potamogeton lucens Linn.）种植区根际沉积物和水中的细菌群落组成，以确定菹草对细菌群落结构的影响。采用 Illumina MiSeq 和靶向 16S rRNA 基因的 qPCR 等分子技术分析了细菌群落的组成和丰度。结果表明，PL 水中的细菌 α 多样性高于 CK 水，而沉积物中的趋势则相反。此外，PL 沉积物中的 16S rRNA 基因拷贝数低于 CK。在沉积物和水中分别发现了 30 个（如 Desulfatiglans）和 29 个（如 Limnohabitans）显著不同的属。菹草可以改变沉积物和水中的化学性质，从而影响细菌群落。在属水平上，细菌群落成员根据来源（水/沉积物）和区域（PL/CK）聚类。本研究表明，沉水植物可以影响沉积物和水中的细菌群落组成，这表明沉水植物影响湖泊生态系统中营养物质的运输和循环。
