金黄色葡萄球菌菌株的生物膜产生分类因所使用的方法而异。
The classification of Staphylococcus aureus strains by biofilm production differs depending on the method used.
机构信息
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañon, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañon, Madrid, Spain.
School of Biology, Universidad Complutense de Madrid, Madrid, Spain.
出版信息
Enferm Infecc Microbiol Clin (Engl Ed). 2022 Mar;40(3):134-137. doi: 10.1016/j.eimce.2020.11.021.
INTRODUCTION
Strains can be classified in terms of biofilm production from quantitative absorbance values collectively by dividing strains into tertile ranks or individually by calculating the optical density for the negative control. However, these methods have not been compared in a large sample of Staphylococcus aureus strains. Therefore, our objective was to analyze the agreement between both methods in terms of biomass production and metabolic activity of their biofilm.
METHODS
We classified 233 S. aureus strains by biomass production and metabolic activity using the crystal violet and XTT assays, respectively. Strains were classified as low, moderate, or high biofilm producers according to tertile or optical density.
RESULTS
We found no agreement between both methods (p<0.001 and p=0.028, respectively).
CONCLUSIONS
We consider strains' biofilm classification by optical density to be a more reliable method, as it depends on the individual absorbance of each strain.
简介
可以根据定量吸光度值将菌株分为三分位等级，或通过计算阴性对照的光密度值对其进行分类，从而从生物膜生产的角度对菌株进行分类。然而，这些方法尚未在大量金黄色葡萄球菌菌株中进行比较。因此，我们的目的是分析这两种方法在生物膜生物量生产和代谢活性方面的一致性。
方法
我们使用结晶紫和 XTT 测定法分别根据生物量生产和代谢活性对 233 株金黄色葡萄球菌菌株进行分类。根据三分位或光密度值，菌株被分类为低、中或高生物膜生产者。
结果
我们发现这两种方法之间没有一致性（分别为 p<0.001 和 p=0.028）。
结论
我们认为，通过光密度对菌株生物膜的分类是一种更可靠的方法，因为它取决于每个菌株的个体吸光度。
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