Wilde C J, Knight C H
Comp Biochem Physiol B. 1986;84(2):197-201. doi: 10.1016/0305-0491(86)90205-1.
Casein degradation was measured by a pulse-chase method in explants from freshly-prepared from goat mammary tissue. An initial increase in L-[5-3H]proline radioactivity incorporated in casein during the chase period was followed in some conditions by a decrease resulting from intracellular degradation of the major casein polypeptides. Differences in the proportion of casein degraded in explants cultured with or without prolactin suggested that the process was under hormonal control. Greater degradation of casein by explants obtained in late pregnancy and early lactation suggested that the process may affect net casein production in vivo.
通过脉冲追踪法测定了从新鲜制备的山羊乳腺组织外植体中酪蛋白的降解情况。在追踪期内，掺入酪蛋白中的L-[5-³H]脯氨酸放射性最初增加，在某些情况下随后会因主要酪蛋白多肽的细胞内降解而降低。在有或没有催乳素的情况下培养的外植体中，酪蛋白降解比例的差异表明该过程受激素控制。妊娠后期和泌乳早期获得的外植体对酪蛋白的降解作用更强，这表明该过程可能会影响体内酪蛋白的净生成。
