Herbert D C, Burke R E, McGuire W L
Cancer Res. 1978 Aug;38(8):2221-3.
Casein and alpha-lactalbumin are characteristic proteins produced by normal mammary cells under hormonal stimulation. Specific antisera were used to immunochemically stain for these two proteins in the unstimulated R3230AC transplantable rat mammary tumor as well as in normal lactating rat mammary tissue. Specific staining was observed in the lactating rat alveolar epithelial cells and in the cells lining the intraalveolar ducts with both antisera. In the R3230AC tumor some cells were shown to contain casein or alpha-lactalbumin; the majority of the tumor cells were unstained. These findings indicate that normal mammary cells as well as a small population of cells within the R3230AC tumor are actively synthesizing casein or alpha-lactalbumin. Furthermore, the results suggest that immunocytochemistry may be used to determine the functional heterogeneity of mammary tumors directly.
酪蛋白和α-乳白蛋白是正常乳腺细胞在激素刺激下产生的特征性蛋白质。使用特异性抗血清对未受刺激的R3230AC可移植大鼠乳腺肿瘤以及正常泌乳大鼠乳腺组织中的这两种蛋白质进行免疫化学染色。两种抗血清在泌乳大鼠肺泡上皮细胞和肺泡内导管内衬细胞中均观察到特异性染色。在R3230AC肿瘤中，一些细胞显示含有酪蛋白或α-乳白蛋白；大多数肿瘤细胞未染色。这些发现表明，正常乳腺细胞以及R3230AC肿瘤内的一小部分细胞正在积极合成酪蛋白或α-乳白蛋白。此外，结果表明免疫细胞化学可直接用于确定乳腺肿瘤的功能异质性。
