Pedersen S, Bloch P L, Reeh S, Neidhardt F C
Cell. 1978 May;14(1):179-90. doi: 10.1016/0092-8674(78)90312-4.
The amount of 140 individual proteins of E. coli B/r was measured during balanced growth in five different media. The abundance of each protein was determined from its absolute amount in 14C-glucose-minimal medium and a measurement of its relative amount at each growth rate using a double labeling technique. Separation of the proteins was carried out by two-dimensional gel electrophoresis. This catalog of proteins, combined with 50 additional ribosomal proteins already studied, comprises about 5% of the coding capacity of the genome, but accounts for two thirds of the cell's protein mass. The behavior of most of these proteins could be described by a relatively small number of patterns. 102 of the 140 proteins exhibited nearly linear variations with growth rate. The remaining 38 proteins exhibited levels which seemed to depend more on the chemical nature of the medium than on growth rate. Proteins, including the ribosomal proteins, that increase in amount with increasing growth rate account for 20% of total cell protein by weight during growth on acetate, 32% on glucose-minimal medium and 55% on glucose-rich medium. Proteins with invariant levels in the various media comprise about 4% of the cell's total protein.
在五种不同培养基中平衡生长期间，对大肠杆菌B/r的140种单个蛋白质的含量进行了测定。每种蛋白质的丰度由其在14C-葡萄糖基本培养基中的绝对含量以及使用双标记技术在每个生长速率下对其相对含量的测量来确定。蛋白质的分离通过二维凝胶电泳进行。这个蛋白质目录，与已经研究的另外50种核糖体蛋白质相结合，约占基因组编码能力的5%，但占细胞蛋白质总量的三分之二。这些蛋白质中的大多数的行为可以用相对较少的模式来描述。140种蛋白质中的102种表现出与生长速率几乎呈线性变化。其余38种蛋白质的含量似乎更多地取决于培养基的化学性质而不是生长速率。在生长过程中，包括核糖体蛋白质在内，随着生长速率增加而含量增加的蛋白质在以乙酸盐为培养基时占细胞总蛋白质重量的20%，在葡萄糖基本培养基中占32%，在富含葡萄糖的培养基中占55%。在各种培养基中含量不变的蛋白质约占细胞总蛋白质的4%。
