Laboratory of Animal Etiology and Epidemiology, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Zhaosu Horse Barn in Yili, Zhaosu, China.
Virus Genes. 2022 Apr;58(2):146-149. doi: 10.1007/s11262-022-01891-y. Epub 2022 Mar 7.
The first representative of a seemingly novel virus family has been found during the metagenomic examination of a diseased and dead horse in the USA [Li et al. in J Gen Virol 96:2721-2733, 2015]. These authors have suggested the need for the establishment of a new family with the tentative name Kirkoviridae; however, the suggested name is not official yet. Soon after the discovery, similar, relatively large CRESS-DNA viruses have been detected in various animals in China and elsewhere. Besides the two main genes (rep and cap), characteristic for members of the family Circoviridae, the tentative kirkoviruses have considerably larger genomes of approximately 4000 nucleotides. Accordingly, these viruses possess three four additional ORFs coding for proteins of unknown function. This has been described previously. In the present manuscript, the authors report the sequence of kirkovirus-like viruses, detected by PCR in donkey excretes in China. From 73 samples, 8 were found positive. From three of these newly detected viruses, the full genomic sequence was determined, whereas from the other five only one gene, namely the replication-associated (Rep) protein was sequenced.
美国在对一匹患病死亡的马进行宏基因组检测时，发现了首个似乎是新型病毒家族的代表[Li 等人，J Gen Virol 96:2721-2733, 2015]。这些作者建议建立一个新的家族，暂定名为 Kirkoviridae；然而，该建议名称尚未正式确定。在发现之后不久，在中国和其他地方的各种动物中也检测到了类似的、相对较大的 CRESS-DNA 病毒。除了两个主要基因（rep 和 cap），这是 Circoviridae 家族成员的特征外，暂定的 Kirkoviruses 具有大约 4000 个核苷酸的更大基因组。因此，这些病毒具有三个额外的 ORF，编码未知功能的蛋白质。这一点以前已经描述过。在本手稿中，作者报告了在中国驴粪便中通过 PCR 检测到的 Kirkovirus 样病毒的序列。在 73 个样本中，有 8 个呈阳性。从其中的 3 个新发现的病毒中，确定了完整的基因组序列，而对另外 5 个病毒仅测序了一个基因，即复制相关（Rep）蛋白。
