Cheng Ye, Li Fan, Xiao Xiaoyue, Zou Shujuan, Chen Jianwei
State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Nanjing Stomatological Hospital, Medical school of Nanjing University, Nanjing, China.
Oral Dis. 2023 May;29(4):1747-1756. doi: 10.1111/odi.14180. Epub 2022 Mar 31.
To investigate the effects of intermittent parathyroid hormone on cementoblast-mediated periodontal repair in the context of orthodontic-induced root resorption.
The rat model of orthodontic-induced root resorption was established. Sixty rats were randomly allocated into the experiment group (n = 30) and the control group (n = 30), either receiving a daily subcutaneous injection of recombinant human PTH or placebo vehicle. Enzyme-linked immunosorbent assay, Micro-computed tomography, hematoxylin and eosin staining, and immunohistochemistry staining were performed to detect the periodontal repair. In vitro, OCCM-30 cells were exposed to intermittent PTH (incubated with PTH for the first 6 h in each 24-h cycle). After three cycles, flow cytometry assay, alkaline phosphatase staining, and Alizarin red staining were performed. Quantitative real-time polymerase chain reaction and Western blotting were employed to further determine the effects of intermittent PTH.
Intermittent PTH-responsive repair enhancement was detected with the expression of bone sialoprotein, osteocalcin, collagen-1, and alkaline phosphatase significantly upregulated. Increased expressions of cementoblastic proteins were positively correlated to cycles of PTH administration. The proportion of cementoblasts in S and G2/M phases was increased; namely, intermittent PTH promoted cementoblast cell proliferation.
Intermittent parathyroid hormone administration promotes cementoblast-mediated cementogenesis during periodontal repair in a time-dependent manner.
研究在正畸诱导牙根吸收背景下，间歇性甲状旁腺激素对成牙骨质细胞介导的牙周组织修复的影响。
建立正畸诱导牙根吸收的大鼠模型。60只大鼠随机分为实验组（n = 30）和对照组（n = 30），分别每日皮下注射重组人甲状旁腺激素或安慰剂。采用酶联免疫吸附测定、显微计算机断层扫描、苏木精-伊红染色和免疫组织化学染色检测牙周组织修复情况。体外实验中，将OCCM-30细胞暴露于间歇性甲状旁腺激素（在每24小时周期的前6小时与甲状旁腺激素共孵育）。三个周期后，进行流式细胞术检测、碱性磷酸酶染色和茜素红染色。采用定量实时聚合酶链反应和蛋白质免疫印迹法进一步确定间歇性甲状旁腺激素的作用。
检测到间歇性甲状旁腺激素反应性修复增强，骨涎蛋白、骨钙素、I型胶原和碱性磷酸酶的表达显著上调。成牙骨质细胞蛋白表达的增加与甲状旁腺激素给药周期呈正相关。S期和G2/M期成牙骨质细胞的比例增加，即间歇性甲状旁腺激素促进成牙骨质细胞增殖。
间歇性给予甲状旁腺激素能以时间依赖性方式促进牙周组织修复过程中成牙骨质细胞介导的牙骨质形成。
