Verrando P, Ortonne J P, Pautrat G, Hsi B L, Yeh C J
J Invest Dermatol. 1986 Aug;87(2):190-6. doi: 10.1111/1523-1747.ep12695328.
A protein which is recognized by an antibody to human amniotic epithelial basement membrane was identified at the basal lamina of human epidermis by immunohistology. This protein was localized at the lamina lucida of human epidermal basement membrane by immunoelectron microscopy. Studies of normal human keratinocyte cultures and epidermal wound healing suggested that the protein was probably produced by keratinocytes. By immunoblotting, a basic apparent isoelectric pH (pHiapp = 7.3) protein band of 37 kD was seen. These data indicate that this 37 kD protein, clearly different from other known basement membrane components, is present in simple and stratified epithelia of ectodermal origin, and is associated with hemidesmosomes.
通过免疫组织学在人表皮基底层鉴定出一种可被抗人羊膜上皮基底膜抗体识别的蛋白质。通过免疫电子显微镜观察，该蛋白质定位于人表皮基底膜的透明层。对正常人角质形成细胞培养物和表皮伤口愈合的研究表明，该蛋白质可能由角质形成细胞产生。通过免疫印迹法，可见一条37 kD的碱性表观等电点（pHiapp = 7.3）蛋白条带。这些数据表明，这种37 kD的蛋白质明显不同于其他已知的基底膜成分，存在于外胚层来源的单层和复层上皮中，并与半桥粒相关。
