Yao Jun, Wang Li, Zhou Hongyan, Xie Zhuang, Zeng Xiang, Liu Chaohui
College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, People's Republic of China; State Key Laboratory of Oil & Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu 610500, People's Republic of China.
College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, People's Republic of China.
J Colloid Interface Sci. 2022 Jun 15;616:858-871. doi: 10.1016/j.jcis.2021.11.103. Epub 2021 Nov 22.
The development of the effective diagnostic method for the determination of cancer biomarkers is one of the most promising strategies for early clinical diagnosis of cancer. Here, based on the preparation of heterogeneous cuprous oxide coated silver (Ag@CuO) nanocomposites/graphitic carbon nitride (g-CN)/cadmium sulfide (CdS) nanoarrays structure, a highly sensitive photoelectrochemical (PEC) biosensor for the examination of carcinoembryonic antigen (CEA) has been constructed successfully. The combination of photoactive semiconductor materials g-CN and CdS increases the electron transfer rate between them and enhances their photocurrent response, thus greatly increasing the concentration detection range. At the same time, the specific recognition between antigen and antibody is used to form a sandwich structure secondary antibody (Ab)/CEA/antibody (Ab). And because Ag@CuO has the function of absorbing light and consuming electron donor. Therefore, the successful measurement of CEA was achieved by labeling Ag@CuO on Ab and finally immobilizing it on the sensor to correlate the current reduction with the CEA concentration. The sandwich PEC biosensor proposed by this signal amplification strategy under optimal conditions has good analytical performance for CEA, with a wide linear detection range (from 10 to 1 ng/mL) and a low detection limit of 0.0011 pg/mL. The PEC biosensor constructed by this method showed high sensitivity, excellent anti-interference ability, favourable repeatability, and good stability.
开发用于测定癌症生物标志物的有效诊断方法是癌症早期临床诊断最具前景的策略之一。在此，基于异质氧化铜包覆银（Ag@CuO）纳米复合材料/石墨相氮化碳（g-CN）/硫化镉（CdS）纳米阵列结构的制备，成功构建了一种用于检测癌胚抗原（CEA）的高灵敏度光电化学（PEC）生物传感器。光活性半导体材料g-CN和CdS的结合提高了它们之间的电子转移速率并增强了它们的光电流响应，从而大大增加了浓度检测范围。同时，利用抗原与抗体之间的特异性识别形成夹心结构二抗（Ab）/CEA/抗体（Ab）。并且由于Ag@CuO具有吸收光和消耗电子供体的功能。因此，通过将Ag@CuO标记在Ab上并最终固定在传感器上，将电流降低与CEA浓度相关联，实现了CEA的成功检测。在最佳条件下，这种信号放大策略提出的夹心PEC生物传感器对CEA具有良好的分析性能，线性检测范围宽（从10到1 ng/mL），检测限低至0.0011 pg/mL。用这种方法构建的PEC生物传感器具有高灵敏度、优异的抗干扰能力、良好的重复性和稳定性。
