Instituto de Procesos Biotecnológicos y Químicos Rosario (IPROBYQ), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CP 2000 Rosario, Argentina.
Instituto de Procesos Biotecnológicos y Químicos Rosario (IPROBYQ), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CP 2000 Rosario, Argentina.
Food Chem. 2022 Aug 1;384:132610. doi: 10.1016/j.foodchem.2022.132610. Epub 2022 Mar 1.
The feasibility of using saponification coupled to extraction with mixed micellar systems to recover bioactive compounds from soybean oil deodorizer distillate, was evaluated for the first time. Under the selected conditions, saponification with KOH 0.6 M and aqueous micellar system prepared with Tergitol 15-S-7 9% w/w, rhamnolipids 0.25 %w/w and sodium citrate 100 mM pH 5.00, at 65 °C, allow the recovery of almost 100% of α- and δ- tocopherols, and 90% of γ-tocopherols. LC-MS measurements demonstrated that the final extract also contained phytosterols and squalene. Additionally, the obtained extract preserved about 100% of the total antioxidant activity. This result was attributable to the fact that 93% of the tocopherols recovered in the micellar phases resulted to be associated with surfactant micelles, environment that is known to improve their antioxidant capacity. These results open perspectives to the use of this methodology to extract these valuable compounds from complex oily sources.
首次评估了使用皂化与混合胶束萃取相结合从大豆油脱臭馏分中回收生物活性化合物的可行性。在选定的条件下，在 65°C 下，用 0.6 M 的 KOH 进行皂化，用 Tergitol 15-S-7（9%w/w）、鼠李糖脂（0.25%w/w）和柠檬酸三钠（100 mM pH 5.00）制备水相胶束体系，可以回收几乎 100%的α-和δ-生育酚，以及 90%的γ-生育酚。LC-MS 测量表明，最终提取物还含有植物甾醇和角鲨烯。此外，所得到的提取物保留了约 100%的总抗氧化活性。这一结果归因于这样一个事实，即在胶束相中回收的 93%的生育酚与表面活性剂胶束有关，众所周知，这种环境可以提高它们的抗氧化能力。这些结果为从复杂的油性来源中提取这些有价值的化合物开辟了新的前景。
