Cho M I, Lee Y L, Garant P R
J Periodontol. 1986 Jul;57(7):413-21. doi: 10.1902/jop.1986.57.7.413.
Ferritin and peroxidase-conjugated antibodies were used in an indirect antibody method to localize fibronectin in gingival connective tissues. Fibronectin was found in the basal lamina beneath the epithelium and endothelium. Collagen fibrils associated with the basement membranes were also heavily coated by fibronectin. Amorphous patches of fibronectin were found adjacent to the plasma membrane of epithelial cells as well as free in the interepithelial spaces. Fibronectin was present throughout the connective tissue in close association with individual collagen fibrils, apparently serving as an interfibrillar cementing substance. Patches of fibronectin were located at the cell surface of fibroblasts, plasma cells, lymphocytes, endothelial cells, smooth muscle cells, and neutrophils. These amorphous patches were observed to connect adjacent cells across narrow spaces and to connect cells to collagen fibrils. The heavy labeling for fibronectin visualized by fluorescent microscopy around gingival blood vessels (Cho et al., 1985) can be accounted for by a heavy coating of fibronectin on the collagen fibrils and basal laminas associated with endothelial cells, as well as by the presence of abundant deposits of fibronectin along the cell membranes of endothelial cells and in the intercellular spaces of the vessel wall.
采用铁蛋白和过氧化物酶偶联抗体的间接抗体法，在牙龈结缔组织中定位纤连蛋白。纤连蛋白存在于上皮和内皮下方的基底层中。与基底膜相关的胶原纤维也被纤连蛋白大量覆盖。在上皮细胞的质膜附近以及上皮间隙中发现了无定形的纤连蛋白斑块。纤连蛋白存在于整个结缔组织中，与单个胶原纤维紧密相连，显然起着纤维间胶结物质的作用。纤连蛋白斑块位于成纤维细胞、浆细胞、淋巴细胞、内皮细胞、平滑肌细胞和中性粒细胞的细胞表面。观察到这些无定形斑块在狭窄空间内连接相邻细胞，并将细胞与胶原纤维相连。荧光显微镜观察到牙龈血管周围纤连蛋白标记强烈（Cho等人，1985年），这可能是由于与内皮细胞相关的胶原纤维和基底层上大量覆盖纤连蛋白，以及内皮细胞膜沿线和血管壁细胞间隙中存在大量纤连蛋白沉积物所致。
