Wariyar Supriya S, Brown Alden D, Tian Tina, Pottorf Tana S, Ward Patricia J
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA.
Department of Biology, Morehouse College, Atlanta, GA, USA.
Exp Neurol. 2022 Jul;353:114029. doi: 10.1016/j.expneurol.2022.114029. Epub 2022 Mar 5.
Enhancing axon regeneration is a major focus of nerve injury research, and the quality of the surgical nerve repair plays a large role in the aggregate success of nerve regeneration. Additionally, exercise is known to promote successful axon regeneration after surgical nerve repair. In this study, we asked how exercise-induced nerve regeneration is affected when a transected nerve is repaired with or without fibrin glue. Fibrin glue repaired nerves exhibited greater vasculature within the tissue bridge compared to nerves that were intrinsically repaired. Fibrin glue repaired nerves also exhibited more robust axon regeneration after exercise compared to nerves that were not repaired with fibrin glue. When angiogenesis of the tissue bridge was prevented, exercise was unable to enhance regeneration despite the presence of fibrin glue. These findings suggest that the biological properties of fibrin glue enhance angiogenesis within the repair site, and a vascularized bridge is required for enhanced axon elongation with exercise. The combination of fibrin glue repair and exercise resulted in notable differences in vascular growth, axon elongation, neuromuscular junction reinnervation, and functional recovery. Fibrin glue should be considered as an adjuvant for nerve repair to enhance the subsequent efficacy of activity- and physical therapy-based treatment interventions.
增强轴突再生是神经损伤研究的一个主要重点，手术神经修复的质量在神经再生的总体成功中起着很大作用。此外，已知运动可促进手术神经修复后的轴突成功再生。在本研究中，我们探讨了在切断的神经使用或不使用纤维蛋白胶修复时，运动诱导的神经再生如何受到影响。与自然修复的神经相比，纤维蛋白胶修复的神经在组织桥内表现出更丰富的血管分布。与未用纤维蛋白胶修复的神经相比，纤维蛋白胶修复的神经在运动后也表现出更强健的轴突再生。当组织桥的血管生成被阻止时，尽管存在纤维蛋白胶，运动也无法增强再生。这些发现表明，纤维蛋白胶的生物学特性可增强修复部位的血管生成，并且血管化的桥对于运动增强轴突伸长是必需的。纤维蛋白胶修复与运动的结合在血管生长、轴突伸长、神经肌肉接头再支配和功能恢复方面产生了显著差异。纤维蛋白胶应被视为神经修复的辅助剂，以提高基于活动和物理治疗的后续治疗干预的疗效。
