Department of Orthopedics, The First Affiliated Hospital of Soochow University, No.188 Shizi St, Suzhou, 215006, Jiangsu Province, P. R. China.
Orthopedic Institute, Medical College, Soochow University, No.708 Renmin Rd, Suzhou, 215006, Jiangsu Province, P. R. China.
BMC Musculoskelet Disord. 2022 Mar 8;23(1):223. doi: 10.1186/s12891-022-05164-5.
This study aims to explore the relationship between surgically-induced ankle instability and posttraumatic osteoarthritis (PTOA) in a mouse model, and to provide reference for clinical practice.
Ligamentectomy was performed on 24 eight-week-old male C57BL/6 J mice, which were divided into three groups. Both the anterior talofibular ligament (ATFL) and the calcaneofibular ligament (CFL) were severed in the CFL + ATFL group, while only the CFL was removed in the CFL group. The SHAM group was set as the blank control group. A wheel-running device was used to accelerate the development of ankle osteoarthritis (OA). Balance measurement, footprint analysis, and histological analysis were used to assess the degree of ankle instability and OA. According to the balance test results, the CFL + ATFL group demonstrated the highest number of slips and the longest crossing beam time at 8 weeks postoperatively. The results of gait analysis exhibited that the CFL + ATFL group had the most significant asymmetry in stride length, stance length, and foot base width compared to the CFL and SHAM groups. The OARSI score of the CFL + ATFL group (16.7 ± 2.18) was also much higher than those of the CFL group (5.1 ± 0.96) and the SHAM group (1.6 ± 1.14).
Based on the mouse model, the findings indicate that severe ankle instability has nearly three times the chance to develop into ankle OA compared to moderate ankle instability.
本研究旨在探讨手术诱导的踝关节不稳定与创伤后骨关节炎（PTOA）之间的关系，为临床实践提供参考。
对 24 只 8 周龄雄性 C57BL/6J 小鼠进行韧带切断术，将其分为三组。CFL+ATFL 组同时切断距腓前韧带（ATFL）和跟腓韧带（CFL），CFL 组仅切除 CFL，SHAM 组作为空白对照组。采用轮跑装置加速踝关节骨关节炎（OA）的发展。平衡测量、足迹分析和组织学分析用于评估踝关节不稳定和 OA 的程度。根据平衡测试结果，CFL+ATFL 组在术后 8 周时的滑倒次数最多，穿过横梁的时间最长。步态分析结果表明，与 CFL 和 SHAM 组相比，CFL+ATFL 组的步长、站立时间和足基宽度的不对称性最明显。CFL+ATFL 组的 OARSI 评分（16.7±2.18）也明显高于 CFL 组（5.1±0.96）和 SHAM 组（1.6±1.14）。
基于小鼠模型，研究结果表明，严重的踝关节不稳定发展为踝关节 OA 的可能性几乎是中度踝关节不稳定的三倍。
