Department of Environmental Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Tshwane, South Africa.
Sci Rep. 2022 Mar 8;12(1):3722. doi: 10.1038/s41598-022-07769-1.
Plant metabolites are known as biological compounds that are essential to the growth and development of a plant and have a direct impact on yield and biochemical constituents of plants. For this study, the objective was to conduct primary metabolomics analysis using liquid chromatography mass spectrometry. African horned cucumber fruits were harvested from plants grown under pots experiment (greenhouse, shade net and open field), soil types (loamy soil and sandy loam) and three water stress levels (no water stress-100%-3L, moderate water stress-75%-2L, and severe water stress-35%-1L) during 2017/18 and 2018/19 seasons. Results showed that the treatment of no water stress combined with sandy loam under shade net environment, significantly increased asparagine content from 10 × 10 to 80 × 10 peak intensity. The treatment of no water stress, in combination with sandy loam soil under open field environment increased 4-hydroxyproline from 10 × 10 to 90 × 10 peak intensity compared to other treatments. It can be deduced that the treatment combination of (no water stress and moderate water stress) and all soil types, under greenhouse environment increased most metabolites content of the fruit when compared to other treatments. Therefore, it subsequently has potential to affect fruit quality such as taste and other biochemical constituents.
植物代谢物是指对植物的生长和发育至关重要的生物化合物，它们直接影响植物的产量和生物化学组成。在这项研究中，目的是使用液相色谱-质谱法进行初级代谢组学分析。非洲角瓜果实是从 2017/18 年和 2018/19 年在盆栽实验（温室、遮阳网和露天）、土壤类型（壤土和沙壤土）和三个水分胁迫水平（无水分胁迫-100%-3L、中度水分胁迫-75%-2L 和严重水分胁迫-35%-1L）下生长的植物中收获的。结果表明，在遮阳网环境下，无水分胁迫与沙壤土结合的处理显著增加了天冬酰胺的含量，从天冬酰胺的峰值强度 10×10 增加到 80×10。与其他处理相比，在露天环境下，无水分胁迫与沙壤土结合的处理使 4-羟基脯氨酸的含量从 10×10 增加到 90×10。可以推断，与其他处理相比，在温室环境下，（无水分胁迫和中度水分胁迫）和所有土壤类型的处理组合增加了果实中大多数代谢物的含量，因此可能会影响果实的品质，如口感和其他生化成分。
