Noe B D, Andrews P C
Peptides. 1986 Mar-Apr;7(2):331-6. doi: 10.1016/0196-9781(86)90232-9.
Sequence analyses of cDNAs prepared from anglerfish islet mRNA have demonstrated the presence of mRNAs coding for two different preproglucagons, aPPG-I and aPPG-II. Each of these precursors was predicted to contain 29 residue and 34 residue glucagon-related peptides as potential cleavage products. Recently, several glucagon-related peptides found in extracts of anglerfish islets have been isolated and characterized. In order to determine whether any of these peptides could be identified as metabolic cleavage products in anglerfish islets, differentially radiolabeled Mr 2,500-8,000 peptides from islet extracts were subjected to reverse phase HPLC under varying conditions. The potential cleavage products aPPG-II[52-80] and aPPG-II[89-122] could be readily identified among the extract peptides. Both peptides became labeled appropriately (as predicted from their sequences) with 13 different amino acids and demonstrated glucagon-like immunoreactivity in a radioimmunoassay. Conversely, a third peptide (aPPG-II[89-119]) could be found among the labeled products in small amounts only. These results demonstrate that glucagon-II[52-80] and aGLP-II[89-112] are primary cleavage products of aPPG-II and suggest that aGLP-IIc[89-119] may be a peptide generated more slowly by post-translational modification of aGLP-II.
对从安康鱼胰岛mRNA制备的cDNA进行的序列分析表明，存在编码两种不同前胰高血糖素原（aPPG-I和aPPG-II）的mRNA。预计这些前体中的每一种都含有29个残基和34个残基的胰高血糖素相关肽作为潜在的裂解产物。最近，在安康鱼胰岛提取物中发现的几种胰高血糖素相关肽已被分离和鉴定。为了确定这些肽中是否有任何一种可以被鉴定为安康鱼胰岛中的代谢裂解产物，将来自胰岛提取物的经差异放射性标记的2500 - 8000道尔顿的肽在不同条件下进行反相高效液相色谱分析。在提取物肽中可以很容易地鉴定出潜在的裂解产物aPPG-II[52 - 80]和aPPG-II[89 - 122]。这两种肽都被13种不同的氨基酸适当地标记（根据它们的序列预测），并在放射免疫分析中显示出胰高血糖素样免疫反应性。相反，第三种肽（aPPG-II[89 - 119]）仅在少量标记产物中被发现。这些结果表明，胰高血糖素-II[52 - 80]和aGLP-II[89 - 112]是aPPG-II的主要裂解产物，并表明aGLP-IIc[89 - 119]可能是由aGLP-II的翻译后修饰产生得更慢的一种肽。
