Shams Azar, Shabani Ronak, Asgari Hamidreza, Karimi Mahdi, Najafi Mohammad, Asghari-Jafarabadi Mohammad, Razavi Seyed Mohsen, Miri Seyed Rouhollah, Abbasi Mehdi, Mohammadi Amirhossein, Koruji Morteza
Stem Cell & Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Nanomedicine (Lond). 2022 Apr;17(8):531-545. doi: 10.2217/nnm-2021-0210. Epub 2022 Mar 10.
MiRNA's-143 and -206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- and 206-loaded poly(lactic--glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. The therapy was conducted in six groups: treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable.
微小RNA-143和-206是癌细胞中强大的凋亡调节因子。本研究旨在使用负载微小RNA-143和-206的聚乳酸-乙醇酸共聚物（PLGA）纳米颗粒与叶酸偶联，以诱导EL4癌细胞凋亡。该治疗在六组中进行：同时用两种微小RNA治疗（混合微小RNA）、微小RNA-206治疗、微小RNA-143治疗、空白PLGA、空白聚乙烯亚胺（PEI）和PEI-微小RNA复合物。在细胞活力方面，在混合微小RNA组中未观察到对EL4细胞清除的协同作用。然而，在单一微小RNA-206组中，观察到比混合微小RNA组和单一微小RNA-143组更强的凋亡效应。发现微小RNA在癌细胞中的凋亡诱导作用显著。
