Department of Perinatology and Gynaecology, Polish Mother's Memorial Hospital Research Institute of Lodz, Lodz, Poland.
Department of Pathology, Chair of Oncology, Medical University of Lodz, Lodz, Poland.
J Obstet Gynaecol. 2022 Aug;42(6):2248-2254. doi: 10.1080/01443615.2022.2037124. Epub 2022 Mar 9.
The aim of the study was to assess immunohistochemical CD34, podoplanin and Ki-67 expression in cervical tumour of patients with cervical squamous cell carcinoma (SCC) staged IIB and IIIB, a relationship with selected clinical and histological parameters and its prognostic significance. This prospective study included 52 patients. Microvessel density (MVD) by CD34, lymphatic vessel density (LVD) by podoplanin and the Ki-67 index in specimens from paraffin blocks with cervical SCC tissues were examined. The relationship between these data and selected clinical and histological parameters was analysed. Positive correlation of MVD and the Ki-67 index was observed. No correlation was observed for MVD, LVD and the Ki-67 index in the tumour with staging, grading, length of treatment and squamous cell carcinoma antigen (SCC-Ag) concentration before and after treatment. The expression of MVD, LVD and the Ki-67 index in cervical SCC did not contribute to the risk of relapse and cancer-related death. No relationship was found for MVD, LVD and the Ki-67 index in cervical tumour of patients with locally advanced cervical SCC with staging, grading and serum SCC-Ag level. MVD, LVD and the Ki-67 index in the tumour did not contribute to the risk of relapse or cervical SCC-related death.Impact Statement In many patients, invasive cervical squamous cell carcinoma (SCC) is diagnosed in a locally advanced stage, when the prognosis depends on many well-known factors connected with tumour biology, staging and general condition of the patient. Despite numerous studies, the value of immunohistochemical CD34, podoplanin and Ki-67 expression in cervical tumour of these patients is still not well defined. In our prospective study, no relationship for microvessel density (MVD), lymphatic vessel density (LVD) and the Ki-67 index in cervical tumour of patients with locally advanced cervical SCC with staging, grading and serum squamous cell carcinoma antigen (SCC-Ag) level was found. Additionally, MVD, LVD and the Ki-67 index in the tumour did not contribute to the risk of relapse or cervical SCC-related death. Our study underlines the limited value of immunohistochemical CD34, podoplanin and Ki-67 expression in cervical tumour of patients with locally advanced cervical SCC. Further research should be focussed on identifying and validating novel prognostic and predictive factors.
本研究旨在评估 CD34、podoplanin 和 Ki-67 的免疫组织化学表达在宫颈鳞癌（SCC）IIB 和 IIIB 期患者的宫颈肿瘤中的作用，以及与选定的临床和组织学参数的关系及其预后意义。这项前瞻性研究纳入了 52 名患者。检查了石蜡块中宫颈 SCC 组织的 CD34 微血管密度（MVD）、podoplanin 淋巴管密度（LVD）和 Ki-67 指数。分析了这些数据与选定的临床和组织学参数之间的关系。观察到 MVD 和 Ki-67 指数之间存在正相关。在肿瘤分期、分级、治疗长度以及治疗前后的鳞状细胞癌抗原（SCC-Ag）浓度方面，MVD、LVD 和 Ki-67 指数之间没有相关性。宫颈 SCC 中 MVD、LVD 和 Ki-67 指数的表达与复发和癌症相关死亡的风险无关。在局部晚期宫颈 SCC 患者的宫颈肿瘤中，MVD、LVD 和 Ki-67 指数与分期、分级和血清 SCC-Ag 水平之间没有关系。肿瘤中的 MVD、LVD 和 Ki-67 指数与复发或宫颈 SCC 相关死亡的风险无关。
影响陈述 在许多患者中，浸润性宫颈鳞癌（SCC）被诊断为局部晚期，此时预后取决于许多与肿瘤生物学、分期和患者一般状况相关的已知因素。尽管进行了大量研究，但这些患者宫颈肿瘤中免疫组织化学 CD34、podoplanin 和 Ki-67 表达的价值仍未得到明确界定。在我们的前瞻性研究中，局部晚期宫颈 SCC 患者的宫颈肿瘤中，MVD、LVD 和 Ki-67 指数与分期、分级和血清 SCC-Ag 水平之间没有关系。此外，肿瘤中的 MVD、LVD 和 Ki-67 指数与复发或宫颈 SCC 相关死亡的风险无关。我们的研究强调了免疫组织化学 CD34、podoplanin 和 Ki-67 表达在局部晚期宫颈 SCC 患者宫颈肿瘤中的有限价值。进一步的研究应集中在确定和验证新的预后和预测因素上。
