Xuzhou Medical University, Xuzhou, Jiangsu, China.
Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
Mol Genet Genomic Med. 2022 May;10(5):e1914. doi: 10.1002/mgg3.1914. Epub 2022 Mar 10.
In recent years, the development of high-throughput sequencing technology has promoted the rapid development of circRNA-related research. Studies have found that circRNA plays a key role in a variety of tumors, but few people study the role of circRNA in nasopharyngeal carcinoma. Under comprehensive treatments, the 5-year survival rate can reach about 70%, but some patients still have distant metastases or recurrences after treatment. Therefore, it is very important to study the molecular mechanisms of the proliferation and invasion of nasopharyngeal carcinoma.
QRT-PCR was applied to detect the relative expression level of circFOXM1 in NPC and nasopharyngeal epithelial cell lines. We knocked down circFOXM1 and studied the influence of circFOXM1 on NPC cells. Nuclear and cytoplasmic RNA isolation experiments, fluorescence in situ hybridization (FISH), bioinformatics analysis, the dual-luciferase reporter experiment, Western Blot, and other experiments were conducted to verify the relationships among circFOXM1, miR-136-5p, and SMAD2. We collected clinical NPC samples to prove the effect of circFOXM1 on the prognosis and treatment of NPC.
In this study, we found that circFOXM1 is highly expressed in nasopharyngeal carcinoma tissue cells compared with adjacent normal tissues and is related to the staging of nasopharyngeal carcinoma. High expression of circFOXM1 indicates a poor prognosis for nasopharyngeal carcinoma. Knockdown of CircFOXM1 inhibited the proliferation and invasion of nasopharyngeal carcinoma cells.
CircFOXM1 promotes the malignant proliferation of nasopharyngeal carcinoma cells by regulating the miR-136-5p-SMAD2 axis.
近年来，高通量测序技术的发展推动了 circRNA 相关研究的快速发展。研究发现 circRNA 在多种肿瘤中发挥关键作用，但很少有人研究 circRNA 在鼻咽癌中的作用。在综合治疗下，5 年生存率可达 70%左右，但部分患者治疗后仍有远处转移或复发。因此，研究鼻咽癌增殖和侵袭的分子机制非常重要。
应用 QRT-PCR 检测 NPC 和鼻咽上皮细胞系中 circFOXM1 的相对表达水平。敲低 circFOXM1 并研究 circFOXM1 对 NPC 细胞的影响。进行核和细胞质 RNA 分离实验、荧光原位杂交（FISH）、生物信息学分析、双荧光素酶报告实验、Western blot 等实验，验证 circFOXM1、miR-136-5p 和 SMAD2 之间的关系。收集临床 NPC 样本，证明 circFOXM1 对 NPC 预后和治疗的影响。
在本研究中，我们发现与相邻正常组织相比，circFOXM1 在鼻咽癌组织细胞中高表达，与鼻咽癌的分期有关。circFOXM1 高表达提示鼻咽癌预后不良。敲低 CircFOXM1 抑制鼻咽癌细胞的增殖和侵袭。
CircFOXM1 通过调节 miR-136-5p-SMAD2 轴促进鼻咽癌细胞的恶性增殖。
