Chen Hui, Shen Xin, Ying Yiping, Li Xiang, Chen Lili, Shen Chensi, Wen Yuezhong
College of Science and Technology, Ningbo University, Cixi 315302, China.
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China.
Environ Sci Process Impacts. 2022 Apr 21;24(4):576-585. doi: 10.1039/d1em00521a.
Copper sulfate (CuSO) is widely used in the control of algal blooms. Cu can promote or inhibit algal growth, while also affecting trace element uptake, therefore, the response mechanisms of algae cells under Cu interference should be studied. In this study, wild-type () and wall-less mutant were selected as the research objects. Except for the cell wall, these two algae were physiologically the same. While manipulating the concentration of Cu, the accumulation of Cu, Fe, Zn, and Mn by the two algal cell types was studied. The cell wall hindered the accumulation of Cu by cells and alleviated the toxicity of Cu to . The addition of Cu increased the accumulation of Fe by both cell types. In an environment with excess Cu, the total amount of Zn and Mn accumulated by cells also increased. On the one hand, this may be due to the synergistic and antagonistic effects of trace elements in the adsorption and uptake process, and on the other hand, it may be due to the changes in metal speciation in the culture medium. In addition, the difference in the total accumulation of various trace elements between wild-type and wall-less-type may be due to the structure and function differences between cell wall and cell membrane. At the same time, by measuring the changes in the levels of glutathione (GSH) in algal cells, the relevant mechanisms underlying the algae's uptake of trace elements by algae were further explored.
硫酸铜（CuSO）被广泛用于控制藻华。铜既能促进也能抑制藻类生长，同时还会影响微量元素的吸收，因此，应该研究藻类细胞在铜干扰下的响应机制。在本研究中，选择野生型（）和无壁突变体作为研究对象。除细胞壁外，这两种藻类在生理上是相同的。在控制铜浓度的同时，研究了两种藻类细胞类型对铜、铁、锌和锰的积累情况。细胞壁阻碍了细胞对铜的积累，并减轻了铜对的毒性。添加铜增加了两种细胞类型对铁的积累。在铜过量的环境中，细胞积累的锌和锰总量也增加了。一方面，这可能是由于微量元素在吸附和吸收过程中的协同和拮抗作用，另一方面，这可能是由于培养基中金属形态的变化。此外，野生型和无壁型之间各种微量元素总积累量的差异可能是由于细胞壁和细胞膜的结构和功能差异。同时，通过测量藻类细胞中谷胱甘肽（GSH）水平的变化，进一步探索了藻类吸收微量元素的相关机制。
