de Waal R M, Bogman M J, Cornelissen I M, Vermeulen A N, Koene R A
Transplantation. 1986 Aug;42(2):178-83. doi: 10.1097/00007890-198608000-00015.
The expression of a class I MHC antigen on the vascular endothelium of mouse skin allografts was assessed by in vivo uptake of radiolabeled monoclonal anti-class-I antibody in the grafts after i.v. injection into the recipients. Endothelial localization of the bound antibodies was demonstrated via double-labeling immunofluorescence microscopy using factor-VIII-related antigen as a marker for endothelial cells. Treatment of recipients with cyclosporine was accompanied by low levels of class I antigen expression in the grafts, and similarly low levels were measured in grafts carried by nude recipients in the complete absence of rejection. Withdrawal of immunosuppressive therapy was followed by an increased class I antigen expression in the donor skin. An increase was also observed in skin grafts undergoing first-set rejection. We conclude that the expression of class I antigens on the capillary endothelium of mouse skin allografts in vivo is variable and is under influence of the immune status of the recipient.
通过静脉注射到受体后，在移植物中对放射性标记的抗I类单克隆抗体进行体内摄取，评估小鼠皮肤同种异体移植物血管内皮上I类MHC抗原的表达。使用因子VIII相关抗原作为内皮细胞的标志物，通过双标记免疫荧光显微镜证实结合抗体的内皮定位。用环孢素治疗受体后，移植物中I类抗原表达水平较低，同样，在完全没有排斥反应的裸鼠受体所携带的移植物中也检测到低水平的I类抗原。停止免疫抑制治疗后，供体皮肤中I类抗原表达增加。在经历初次排斥反应的皮肤移植物中也观察到增加。我们得出结论，小鼠皮肤同种异体移植物毛细血管内皮上I类抗原的表达在体内是可变的，并受受体免疫状态的影响。
