Akintayo Damilola C, de la Torre Beatriz G, Li Yongfu, Albericio Fernando
Peptide Science Laboratory, School of Chemistry and Physics, University of KwaZulu-Natal, Westville, Durban 4000, South Africa.
KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa.
Polymers (Basel). 2022 Feb 25;14(5):928. doi: 10.3390/polym14050928.
Amino-Li-resin is a new and unique polyacrylamide resin presented in the form of fibers and is found to be well suited for solid-phase peptide chemistry. Although amino-Li-resin swells much better in polar solvents, it is also compatible with some non-polar solvents. It comes with a high loading of functional amino groups, thus maximizing its productivity in terms of the amount of peptide per gram of resin. In addition to its mechanical stability, this resin shows excellent stability in basic and acidic reagents; thus, allowing its broad applicability for the synthesis of a wide range of biomolecules. Finally, the appropriateness of amino-Li-resin for solid-phase peptide synthesis (SPPS) has been demonstrated for the synthesis of several model peptides, including difficult sequences and those containing hindered amino acids, all of which afforded excellent crude purity, as shown by high-performance liquid chromatography (HPLC) analysis.
氨基锂树脂是一种新型独特的聚丙烯酰胺树脂，呈纤维状，被发现非常适合用于固相肽化学。尽管氨基锂树脂在极性溶剂中的溶胀性要好得多，但它也与一些非极性溶剂兼容。它带有高负载量的官能氨基，因此就每克树脂的肽量而言，能使其生产率最大化。除了具有机械稳定性外，这种树脂在碱性和酸性试剂中也表现出优异的稳定性；因此，使其在合成多种生物分子方面具有广泛的适用性。最后，通过高效液相色谱（HPLC）分析表明，氨基锂树脂适用于固相肽合成（SPPS），已被用于合成几种模型肽，包括具有困难序列的肽以及含有受阻氨基酸的肽，所有这些肽都具有优异的粗品纯度。
