Korea Institute of Brain Science, Seoul 06022, Korea.
Temasek Life Sciences Laboratories, Singapore 117604, Singapore.
Nutrients. 2022 Mar 5;14(5):1098. doi: 10.3390/nu14051098.
(DT) is a medicinal herb native to East Asia, which has been used extensively for its therapeutic properties in traditional medicine. In this study, we examined the effects of DT sap on the regulation of body weight and muscle metabolism in mice. Obese model db/db mice were administered daily with DT sap or vehicle control over a 6-week period. The effects of DT sap on muscle metabolism were studied in C2C12 muscle cells, where glycolytic and mitochondrial respiration rates were monitored. As AMP-activated protein kinase (AMPK) is a master regulator of metabolism and plays an important function as an energy sensor in muscle tissue, signaling pathways related with AMPK were also examined. We found that DT sap inhibited body weight increase in db/db, db/+, and +/+ mice over a 6-week period, while DT sap-treated muscle cells showed increased muscle metabolism and also increased phosphorylation of AMPK and Acetyl-CoA Carboxylase (ACC). Finally, we found that DT sap, which is enriched in estrogen in our previous study, significantly activates estrogen alpha receptor in a concentration-dependent manner, which can drive the activation of AMPK signaling and may be related to the muscle metabolism and weight changes observed here.
(DT) 是一种原产于东亚的药用草本植物，在传统医学中因其治疗特性而被广泛应用。在这项研究中，我们研究了 DT 树液对肥胖模型 db/db 小鼠体重调节和肌肉代谢的影响。db/db 肥胖模型小鼠每天给予 DT 树液或对照载体处理，持续 6 周。在 C2C12 肌肉细胞中研究了 DT 树液对肌肉代谢的影响，监测糖酵解和线粒体呼吸速率。由于 AMP 激活的蛋白激酶 (AMPK) 是代谢的主要调节剂，并且在肌肉组织中作为能量传感器发挥重要功能，因此还检查了与 AMPK 相关的信号通路。我们发现，在 6 周的时间里，DT 树液抑制了 db/db、db/+ 和 +/+ 小鼠的体重增加，而 DT 树液处理的肌肉细胞显示出肌肉代谢增加，并且 AMPK 和乙酰辅酶 A 羧化酶 (ACC) 的磷酸化也增加。最后，我们发现，我们之前的研究表明 DT 树液富含雌激素，它以浓度依赖的方式显著激活雌激素受体 α，这可能会驱动 AMPK 信号的激活，并且可能与我们在这里观察到的肌肉代谢和体重变化有关。
