CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Molecules. 2022 Feb 28;27(5):1618. doi: 10.3390/molecules27051618.
Liquid chromatography plays a central role in biomanufacturing, and, apart from its use as a preparative purification strategy, either in biopharmaceuticals or in fine chemicals industries, it is also very useful as an analytical tool for monitoring, assessing, and characterizing diverse samples. The present review gives an overview of the progress of the chromatographic supports that have been used in the purification of high-value products (e.g., small molecules, organic compounds, proteins, and nucleic acids). Despite the diversity of currently available chromatographic matrices, the interest in innovative biomolecules emphasizes the need for novel, robust, and more efficient supports and ligands with improved selectivity. Accordingly, ionic liquids (ILs) have been investigated as novel ligands in chromatographic matrices. Given herein is an extensive review regarding the different immobilization strategies of ILs in several types of supports, namely in silica, Sepharose, and polymers. In addition to depicting their synthesis, the main application examples of these supports are also presented. The multiple interactions promoted by ILs are critically discussed concerning the improved selectivity towards target molecules. Overall, the versatility of supported ILs is here considered a critical point to their exploitation as alternatives to the more conventional liquid chromatographic matrices used in bioseparation processes.
液相色谱在生物制造中起着核心作用，除了作为制备性纯化策略（无论是在生物制药还是精细化工行业）之外，它还是一种非常有用的分析工具，可用于监测、评估和表征各种样品。本综述概述了用于纯化高价值产品（例如小分子、有机化合物、蛋白质和核酸）的色谱支撑体的进展。尽管目前可用的色谱基质种类繁多，但对创新生物分子的兴趣强调了需要新型、稳健且更有效的支撑体和配体，以提高选择性。因此，离子液体 (IL) 已被研究作为色谱基质中的新型配体。本文对 IL 在几种类型的支撑体（即硅胶、Sepharose 和聚合物）中的不同固定化策略进行了广泛的综述。除了描述它们的合成之外，还介绍了这些支撑体的主要应用实例。还对 IL 促进的多种相互作用进行了批判性讨论，以了解它们对目标分子的选择性提高。总的来说，被支持的 IL 的多功能性被认为是将其作为生物分离过程中使用的更传统的液相色谱基质的替代品的一个关键点。
