Notario Andrés, Sánchez Rosario, Luaces Pilar, Sanz Carlos, Pérez Ana G
Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, Campus UPO, Ctra. Utrera km 1, Bldg. 46, 41013 Seville, Spain.
Molecules. 2022 Mar 2;27(5):1650. doi: 10.3390/molecules27051650.
, the olive fruit fly, is one of the most important pests affecting the olive fruit, causing serious quantitative and qualitative damage to olive oil production. In this study, the changes induced by infestation in the biosynthesis of volatile and phenolic compounds in olive (cvs. Picual, Manzanilla, and Hojiblanca) have been analyzed. Despite cultivar differences, the oils obtained from infested fruits showed a significant increase in the content of certain volatile compounds such as (-hex-2-enal, ethanol, ethyl acetate, and β-ocimene and a drastic decrease of the phenolic contents. The impact of those changes on the inferred quality of the oils has been studied. In parallel, the changes induced by the attack of the olive fly on the expression of some key genes in the biosynthesis of volatile and phenolic compounds, such as lipoxygenase, β-glucosidase, and polyphenol oxidase, have been analyzed. The strong induction of a new olive polyphenol oxidase gene ( explains the reduction of phenolic content in the oils obtained from infested fruits and suggest the existence of a PPO-mediated oxidative defense system in olives.
地中海实蝇是影响橄榄果实的最重要害虫之一，对橄榄油生产造成严重的数量和质量损害。在本研究中，分析了侵染对橄榄（品种Picual、Manzanilla和Hojiblanca）中挥发性和酚类化合物生物合成的影响。尽管品种存在差异，但从受侵染果实中获得的油中，某些挥发性化合物如（-己烯醛、乙醇、乙酸乙酯和β-罗勒烯的含量显著增加，而酚类含量则急剧下降。研究了这些变化对推断的油品质的影响。同时，分析了橄榄实蝇攻击对挥发性和酚类化合物生物合成中一些关键基因（如脂氧合酶、β-葡萄糖苷酶和多酚氧化酶）表达的影响。一种新的橄榄多酚氧化酶基因的强烈诱导（解释了从受侵染果实中获得的油中酚类含量的降低，并表明橄榄中存在PPO介导的氧化防御系统。
