Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia.
Department of Maxillofacial Surgery, Medical Faculty Pristina in Kosovska Mitrovica, 38220 Kosovska Mitrovica, Serbia.
Molecules. 2022 Mar 4;27(5):1694. doi: 10.3390/molecules27051694.
Reconstruction of jaw bone defects present a significant problem because of specific aesthetic and functional requirements. Although widely used, the transplantation of standard autograft and allograft materials is still associated with significant constraints. Composite scaffolds, combining advantages of biodegradable polymers with bioceramics, have potential to overcome limitations of standard grafts. Polyethyleneimine could be an interesting novel biocompatible polymer for scaffold construction due to its biocompatibility and chemical structure. To date, there have been no in vivo studies assessing biological properties of hydroxyapatite bioceramics scaffold modified with polyethyleneimine. The aim of this study was to evaluate in vivo effects of composite scaffolds of hydroxyapatite ceramics and poly(lactide-co-glycolide) and novel polyethyleneimine on bone repair in swine's mandibular defects, and to compare them to conventional bone allograft (BioOss). Scaffolds were prepared using the method of polymer foam template in three steps. Pigs, 3 months old, were used and defects were made in the canine, premolar, and molar area of their mandibles. Four months following the surgical procedure, the bone was analyzed using radiological, histological, and gene expression techniques. Hydroxyapatite ceramics/polyethyleneimine composite scaffold demonstrated improved biological behavior compared to conventional allograft in treatment of swine's mandibular defects, in terms of bone density and bone tissue histological characteristics.
颌骨缺损的重建是一个重大问题，因为这涉及到特定的美学和功能要求。尽管广泛应用，但标准自体移植物和同种异体移植物材料的移植仍然存在很大的局限性。复合支架将可生物降解聚合物与生物陶瓷的优势结合在一起，具有克服标准移植物局限性的潜力。聚亚乙基亚胺由于其生物相容性和化学结构，可能成为支架构建的一种有趣的新型生物相容聚合物。迄今为止，还没有体内研究评估过用聚亚乙基亚胺改性的羟磷灰石生物陶瓷支架的生物特性。本研究旨在评估羟磷灰石陶瓷和聚（乳酸-共-乙醇酸）与新型聚亚乙基亚胺复合支架对猪下颌骨缺损的体内修复作用，并将其与传统的同种异体骨（BioOss）进行比较。支架通过三步聚合物泡沫模板法制备。使用 3 个月大的猪，在它们的下颌骨的犬齿、前磨牙和磨牙区域制造缺损。在手术 4 个月后，使用放射学、组织学和基因表达技术对骨骼进行分析。与传统同种异体骨相比，羟磷灰石陶瓷/聚亚乙基亚胺复合支架在治疗猪下颌骨缺损方面表现出更好的生物学行为，表现在骨密度和骨组织的组织学特征方面。
