Liu Lu, Shi Guixin, Cui Ying, Li Hui, Li Zhengchao, Zeng Qiangcheng, Guo Yong
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Biomed Tech (Berl). 2017 Oct 26;62(5):467-479. doi: 10.1515/bmt-2016-0005.
The construction of engineered bone mostly focuses on simulating the extracellular matrix (ECM) for proper biological activity. However, the complexity of architecture and the variability of the mechanical properties of natural bones are related to individual differences in age, nutritional state, mechanical loading and disease status. Defect substitutions should be normed with the host natural bone, balancing architectural and mechanical adaption, as well as biological activity. Using a freeform fabrication (FFF) method, we prepared polycaprolactone (PCL) scaffolds with different architectures. With simulation of structural and mechanical parameters of rabbit femur cancellous bone, individual defect substitution with the characteristics of the rabbit femur was obtained with high porosity and connectivity. Biological adaption in vitro was examined and osteoid formation in vivo was assessed by implantation in situ. Simulating the femur cancellous bone, 300-μm FFF PCL scaffolds had better architectural and mechanical properties. The protocol produced an architecturally, mechanically and biologically adaptive construction of an individual model for rapid-prototype PCL scaffolds. A guide system was developed to accurately reproduce virtually individual defect substitutions of the bone.
工程骨的构建主要集中在模拟细胞外基质（ECM）以实现适当的生物活性。然而，天然骨结构的复杂性和力学性能的变异性与年龄、营养状态、机械负荷和疾病状态的个体差异有关。缺损替代物应与宿主天然骨标准化，平衡结构和力学适应性以及生物活性。我们采用自由成型制造（FFF）方法制备了具有不同结构的聚己内酯（PCL）支架。通过模拟兔股骨松质骨的结构和力学参数，获得了具有高孔隙率和连通性的、具有兔股骨特征的个体缺损替代物。检测了体外生物适应性，并通过原位植入评估了体内类骨质形成。模拟股骨松质骨，300μm的FFF PCL支架具有更好的结构和力学性能。该方案为快速原型PCL支架生成了一个在结构、力学和生物学上具有适应性的个体模型构建。开发了一种导向系统，以准确再现骨骼的虚拟个体缺损替代物。
