Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Heye Health Industrial Research Institute, Zhejiang Heye Health Technology, Anji, Huzhou 313300, China.
Cells. 2022 Feb 27;11(5):827. doi: 10.3390/cells11050827.
Magnetobiological effects on growth and virulence have been widely reported in (). However, published results are quite varied and sometimes conflicting because the underlying mechanism remains unknown. Here, we reported that the application of 250 mT static magnetic field (SMF) significantly reduces the diameter of colony-forming units (CFUs) but has no impact on the number of CFUs. Transcriptomic analysis revealed that the inhibitory effect of SMF is attributed to differentially expressed genes (DEGs) primarily involved in carbon source utilization. Consistently, the addition of glycolate or glyoxylate to the culture media successfully restores the bacterial phenotype in SMF, and knockout mutants lacking glycolate oxidase are no longer sensitive to SMF. These results suggest that SMF treatment results in a decrease in glycolate oxidase activity. In addition, metabolomic assay showed that long-chain fatty acids (LCFA) accumulate while phosphatidylglycerol and middle-chain fatty acids decrease in the SMF-treated bacteria, suggesting that SMF inhibits LCFA degradation. Based on the published evidence together with ours derived from this study, we propose a model showing that free radicals generated by LCFA degradation are the primary target of SMF action, which triggers the bacterial oxidative stress response and ultimately leads to growth inhibition.
磁场生物效应在生长和毒力方面已被广泛报道[()]。然而，由于其潜在机制尚不清楚，发表的结果差异很大，有时甚至相互矛盾。在这里，我们报告称，施加 250 mT 静磁场（SMF）会显著减小[()]集落形成单位（CFUs）的直径，但对 CFUs 的数量没有影响。转录组分析显示，SMF 的抑制作用归因于差异表达基因（DEGs），这些基因主要参与碳源的利用。一致地，向培养基中添加乙醇酸盐或乙醛酸盐可成功恢复 SMF 中的细菌表型，而缺乏乙醇酸盐氧化酶的敲除突变体不再对 SMF 敏感。这些结果表明，SMF 处理会导致乙醇酸盐氧化酶活性降低。此外，代谢组学分析表明，在 SMF 处理的细菌中，长链脂肪酸（LCFA）积累，而磷脂酰甘油和中链脂肪酸减少，表明 SMF 抑制 LCFA 降解。基于已发表的证据以及我们从本研究中获得的证据，我们提出了一个模型，表明由 LCFA 降解产生的自由基是 SMF 作用的主要靶标，它触发了细菌的氧化应激反应，最终导致生长抑制。
