一种用于氨基丙基转移酶的快速非色谱检测法。
A rapid nonchromatographic assay for aminopropyltransferases.
作者信息
Anton D L
出版信息
Anal Biochem. 1986 Jul;156(1):45-7. doi: 10.1016/0003-2697(86)90151-x.
PMID:3526973
Abstract
Aminopropyltransferases are key enzymes in the biosynthesis of the polyamines spermidine and spermine. A procedure is described for assaying these enzymes by differential charcoal adsorption of 14C-labeled decarboxylated adenosylmethionine substrate from the labeled polyamine product. This assay is linear with time and enzyme concentration, and is suitable for use with a variety of amine acceptors. This procedure has the advantage, over those previously used, that it is extremely rapid yet very sensitive.
摘要
氨基丙基转移酶是多胺亚精胺和精胺生物合成中的关键酶。本文描述了一种通过从标记的多胺产物中用活性炭差异吸附14C标记的脱羧腺苷甲硫氨酸底物来测定这些酶的方法。该测定方法与时间和酶浓度呈线性关系，适用于多种胺受体。与以前使用的方法相比，该方法具有极其快速且非常灵敏的优点。
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